2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. 2D-PAGE.

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2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. 2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. Cells were transfected with either siScr or siNR4A1 for 60 hours, and whole cell lysates (400 μg of protein) were prepared, and the pH gradient in the first dimension was from 3 to 10. The second dimension was a 12% acrylamide SDS-PAGE. Gels were stained by colloidal staining with Coomassie blue G250 (A). Arrows point to the proteins of interest, and the numbers assigned to the spots correspond to the numbers listed in Supplementary Table S2. Functional distribution (B) and canonical pathway (C) of the 38 identified proteins. Assignments were made based on information from the NCBI, the Swiss-Prot/TrEMBL Protein Knowledge Base, and the Ingenuity Pathways Knowledge Base. Enlarged images of protein spots for which image analysis software reported ≥2-fold differences in accumulation in siNR4A1-transfected cells are shown (D, left). Panc-1 cells were transfected with either siScr or siNR4A1 for 60 hours, and whole cell lysates were analyzed by Western blot analysis (D, right); ER, endoplasmic reticulum. Syng-Ook Lee et al. Mol Cancer Res 2014;12:527-538 ©2014 by American Association for Cancer Research