Distribution of diterpenoids in Stevia leaf tissues.

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Distribution of diterpenoids in Stevia leaf tissues. Distribution of diterpenoids in Stevia leaf tissues. Tissue-specific abundance of metabolites was evaluated by ultra high performance liquid chromatography (A) and GC-MS (B). A, Comparison of SG content between leaf-trichomes and trichomes. Standards contain a mixture of nine known SGs. Peak a, Rebaudioside (Reb) D. Peak b, Reb A. Peak c, stevioside. Peak d, Reb F. Peak e, Reb C. Peak f, dulcoside A. Peak g, rubusoside. Peak h, Reb B. Peak i, steviobioside. mAU, Milliabsorbance units. B, GC traces showing the difference between leaf-trichomes and trichomes. The arrow indicates the peak of camphor (10 µg µL–1), the internal standard used in the assay. The peaks numbered in GC traces were identical to those listed in Table I. Mi Jung Kim et al. Plant Physiol. 2015;169:2462-2480 ©2015 by American Society of Plant Biologists