Schematic representation of the translation and processing strategy of the RV ns and structural proteins. Schematic representation of the translation and processing strategy of the RV ns and structural proteins. The RV genome comprises two long nonoverlapping ORFs, with the 5′ ORF coding for the ns proteins and the 3′ ORF coding for the structural proteins. A polyprotein precursor, p200, is translated from the 5′ ORF of the RV genomic RNA and undergoes cis cleavage to produce two ns proteins, p150 and p90. The locations of the putative amino acid motifs for methyltransferase (M), X motif, papain-like cysteine protease (P), helicase (H), and replicase (R) are indicated on the 5′ ORF. The RV structural proteins are synthesized from a 24S subgenomic RNA transcribed from the 3′ ORF. A polyprotein precursor, p100, is translated from the subgenomic RNA and undergoes several posttranslational modifications to ultimately produce the mature capsid (C), E2, and E1. Jia-Yee Lee, and D. Scott Bowden Clin. Microbiol. Rev. 2000; doi:10.1128/CMR.13.4.571