Γ-H2AX foci formation in ATM−/− and DNA-dependent protein kinase catalytic subunit (DNA-PKcs)−/− mouse embryo fibroblasts (MEFs) but not in ATM−/− MEFs.

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γ-H2AX foci formation in ATM−/− and DNA-dependent protein kinase catalytic subunit (DNA-PKcs)−/− mouse embryo fibroblasts (MEFs) but not in ATM−/− MEFs treated with LY294002. γ-H2AX foci formation in ATM−/− and DNA-dependent protein kinase catalytic subunit (DNA-PKcs)−/− mouse embryo fibroblasts (MEFs) but not in ATM−/− MEFs treated with LY294002. A, time course of γ-H2AX foci formation in MEFs after exposure to 2 Gy of γ-rays. Error bars represent the SD of the mean of three independent experiments. B, foci formation in ATM−/−, DNA-PKcs−/− MEFs and their ablation in ATM−/− MEFS treated with LY294002. Cells were immunostained for γ-H2AX (left panels) and counterstained with 4′,6-diamidino-2-phenylindole (right panels). Tom Stiff et al. Cancer Res 2004;64:2390-2396 ©2004 by American Association for Cancer Research