HLA-C Level Is Regulated by a Polymorphic Oct1 Binding Site in the HLA-C Promoter Region  Nicolas Vince, Hongchuan Li, Veron Ramsuran, Vivek Naranbhai,

Slides:



Advertisements
Similar presentations
A Single SNP in an Evolutionary Conserved Region within Intron 86 of the HERC2 Gene Determines Human Blue-Brown Eye Color  Richard A. Sturm, David L.
Advertisements

by Hanna S. Radomska, Anne B
by Kumar Kotlo, Douglas E. Hughes, Victoria L. M
Serotonin Transporter Promoter Gain-of-Function Genotypes Are Linked to Obsessive- Compulsive Disorder  Xian-Zhang Hu, Robert H. Lipsky, Guanshan Zhu,
An IgE-associated polymorphism in STAT6 alters NF-κB binding, STAT6 promoter activity, and mRNA expression  Michaela Schedel, PhD, Remo Frei, PhD, Christian.
Novel Functional Single Nucleotide Polymorphisms in the Latent Transforming Growth Factor-β Binding Protein-1L Promoter  Tomomi Higashi, Satoru Kyo, Masaki.
by Hong Hao, Huiling Qi, and Manohar Ratnam
Promoter and 3′-Untranslated-Region Haplotypes in the Vitamin D Receptor Gene Predispose to Osteoporotic Fracture: The Rotterdam Study  Yue Fang, Joyce.
Polymorphisms related to ORMDL3 are associated with asthma susceptibility, alterations in transcriptional regulation of ORMDL3, and changes in TH2 cytokine.
Analysis of a Genetic Polymorphism in the Costimulatory Molecule TNFSF4 with Hematopoietic Stem Cell Transplant Outcomes  Peter T. Jindra, Susan E. Conway,
Jodie N. Painter, Susanne Kaufmann, Tracy A. O’Mara, Kristine M
Common Variants of Large Effect in F12, KNG1, and HRG Are Associated with Activated Partial Thromboplastin Time  Lorna M. Houlihan, Gail Davies, Albert.
The homeodomain protein Cdx2 regulates lactase gene promoter activity during enterocyte differentiation  Rixun Fang, Nilda A. Santiago, Lynne C. Olds,
A.-K. Wenke, S. Niebler, S. Grässel, A.K. Bosserhoff 
Functional Variants in the Promoter Region of Chitinase 3–Like 1 (CHI3L1) and Susceptibility to Schizophrenia  Xinzhi Zhao, Ruqi Tang, Bo Gao, Yongyong.
Substance P Enhances the Production of Interferon-induced Protein of 10 kDa by Human Keratinocytes in Synergy with Interferon-γ  Naoko Kanda, Shinichi.
Independent Introduction of Two Lactase-Persistence Alleles into Human Populations Reflects Different History of Adaptation to Milk Culture  Nabil Sabri.
Genome-wide Association and Follow-Up Replication Studies Identified ADAMTS18 and TGFBR3 as Bone Mass Candidate Genes in Different Ethnic Groups  Dong-Hai.
Multiple Hepatic Regulatory Variants at the GALNT2 GWAS Locus Associated with High-Density Lipoprotein Cholesterol  Tamara S. Roman, Amanda F. Marvelle,
Mutation of a Nuclear Respiratory Factor 2 Binding Site in the 5′ Untranslated Region of the ADSL Gene in Three Patients with Adenylosuccinate Lyase Deficiency 
Serotonin Transporter Promoter Gain-of-Function Genotypes Are Linked to Obsessive- Compulsive Disorder  Xian-Zhang Hu, Robert H. Lipsky, Guanshan Zhu,
The interferon regulatory factor ICSBP/IRF-8 in combination with PU
SOX10 Promotes Melanoma Cell Invasion by Regulating Melanoma Inhibitory Activity  Saskia A. Graf, Christian Busch, Anja-Katrin Bosserhoff, Robert Besch,
High-Resolution Genetic Maps Identify Multiple Type 2 Diabetes Loci at Regulatory Hotspots in African Americans and Europeans  Winston Lau, Toby Andrew,
Sp1 Is Required for Glucose-Induced Transcriptional Regulation of Mouse Vesicular Glutamate Transporter 2 Gene  Tao Li, Liqun Bai, Jing Li, Suzu Igarashi,
Rose-Anne Romano, Barbara Birkaya, Satrajit Sinha 
A Single SNP in an Evolutionary Conserved Region within Intron 86 of the HERC2 Gene Determines Human Blue-Brown Eye Color  Richard A. Sturm, David L.
HLX1 gene variants influence the development of childhood asthma
Jason Park, Stephanie Schulz, Scott A. Waldman  Gastroenterology 
Qiujie Jiang, Yasushi Matsuzaki, Kehua Li, Jouni Uitto 
The Structure of Linkage Disequilibrium at the DBH Locus Strongly Influences the Magnitude of Association between Diallelic Markers and Plasma Dopamine.
Weight Loss after Gastric Bypass Is Associated with a Variant at 15q26
Transcriptional Control of the Mouse Col7a1 Gene in Keratinocytes: Basal and Transforming Growth Factor-β Regulated Expression  Michael Naso, Jouni Uitto,
Imputation of Exome Sequence Variants into Population- Based Samples and Blood- Cell-Trait-Associated Loci in African Americans: NHLBI GO Exome Sequencing.
Long-Range Modulation of PAG1 Expression by 8q21 Allergy Risk Variants
Yin-Yang 1 Negatively Regulates the Differentiation-Specific Transcription of Mouse Loricrin Gene in Undifferentiated Keratinocytes  Xuezhu Xu, Yasuhiro.
Single-nucleotide polymorphisms in the promoter region influence the expression of the human follicle-stimulating hormone receptor  Alain Wunsch, M.Sc.,
The β-Globin Recombinational Hotspot Reduces the Effects of Strong Selection around HbC, a Recently Arisen Mutation Providing Resistance to Malaria  Elizabeth.
Integrative Multi-omic Analysis of Human Platelet eQTLs Reveals Alternative Start Site in Mitofusin 2  Lukas M. Simon, Edward S. Chen, Leonard C. Edelstein,
Assessment of the Genetic Basis of Rosacea by Genome-Wide Association Study  Anne Lynn S. Chang, Inbar Raber, Jin Xu, Rui Li, Robert Spitale, Julia Chen,
Integrative Functional Genomics Implicates EPB41 Dysregulation in Hepatocellular Carcinoma Risk  Xinyu Yang, Dianke Yu, Yanli Ren, Jinyu Wei, Wenting.
Genetic Investigations of Kidney Disease: Core Curriculum 2013
RBPJ Mutations Identified in Two Families Affected by Adams-Oliver Syndrome  Susan J. Hassed, Graham B. Wiley, Shaofeng Wang, Ji-Yun Lee, Shibo Li, Weihong.
Volume 60, Issue 3, Pages (September 2001)
Transcriptional Regulation of ATP2C1 Gene by Sp1 and YY1 and Reduced Function of its Promoter in Hailey–Hailey Disease Keratinocytes  Hiroshi Kawada,
Haplotypes at ATM Identify Coding-Sequence Variation and Indicate a Region of Extensive Linkage Disequilibrium  Penelope E. Bonnen, Michael D. Story,
Histamine Inhibits the Production of Interferon-induced Protein of 10 kDa in Human Squamous Cell Carcinoma and Melanoma  Naoko Kanda, Shinichi Watanabe 
Naoko Kanda, Shinichi Watanabe  Journal of Investigative Dermatology 
A Three–Single-Nucleotide Polymorphism Haplotype in Intron 1 of OCA2 Explains Most Human Eye-Color Variation  David L. Duffy, Grant W. Montgomery, Wei.
Cyclooxygenase-2 Inhibitor Enhances Whereas Prostaglandin E2Inhibits the Production of Interferon-Induced Protein of 10 kDa in Epidermoid Carcinoma A431 
Keratinocyte growth factor promotes goblet cell differentiation through regulation of goblet cell silencer inhibitor  Dai Iwakiri, Daniel K. Podolsky 
Are Interactions between cis-Regulatory Variants Evidence for Biological Epistasis or Statistical Artifacts?  Alexandra E. Fish, John A. Capra, William.
Cowden Syndrome–Affected Patients with PTEN Promoter Mutations Demonstrate Abnormal Protein Translation  Rosemary E. Teresi, Kevin M. Zbuk, Marcus G.
Mutations in the DBP-Deficiency Protein HSD17B4 Cause Ovarian Dysgenesis, Hearing Loss, and Ataxia of Perrault Syndrome  Sarah B. Pierce, Tom Walsh, Karen.
The G/G Genotype of a Resistin Single-Nucleotide Polymorphism at −420 Increases Type 2 Diabetes Mellitus Susceptibility by Inducing Promoter Activity.
Genome-wide Association Study Reveals Multiple Nasopharyngeal Carcinoma- Associated Loci within the HLA Region at Chromosome 6p21.3  Ka-Po Tse, Wen-Hui.
Regulation of the Expression of Peptidylarginine Deiminase Type II Gene (PADI2) in Human Keratinocytes Involves Sp1 and Sp3 Transcription Factors  Sijun.
Identification of Rare, Disease-Associated Variants in the Promoter Region of the RNF114 Psoriasis Susceptibility Gene  Alexandros Onoufriadis, Michael.
Volume 7, Issue 6, Pages (December 1997)
IFN-γ Represses IL-4 Expression via IRF-1 and IRF-2
A Promoter Sequence Variant of ZNF750 Is Linked with Familial Psoriasis  Chi-Fan Yang, Wuh-Liang Hwu, Li-Cheng Yang, Wen-Hung Chung, Yin-Hsiu Chien, Chia-Fu.
Defining the Regulatory Elements in the Proximal Promoter of ΔNp63 in Keratinocytes: Potential Roles for Sp1/Sp3, NF-Y, and p63  Rose-Anne Romano, Barbara.
Naoko Kanda, Shinichi Watanabe  Journal of Investigative Dermatology 
Bart A. Jessen, Marjorie A. Phillips, Robert H. Rice 
Common Variants of Large Effect in F12, KNG1, and HRG Are Associated with Activated Partial Thromboplastin Time  Lorna M. Houlihan, Gail Davies, Albert.
Deon G. Uffort, Elizabeth A. Grimm, Julie A. Ellerhorst 
NF-kB binds to the NF-κB binding site immediately adjacent to the AT-rich regulatory region. NF-kB binds to the NF-κB binding site immediately adjacent.
Volume 16, Issue 5, Pages (December 2004)
Volume 129, Issue 2, Pages (August 2005)
Beyond GWASs: Illuminating the Dark Road from Association to Function
Presentation transcript:

HLA-C Level Is Regulated by a Polymorphic Oct1 Binding Site in the HLA-C Promoter Region  Nicolas Vince, Hongchuan Li, Veron Ramsuran, Vivek Naranbhai, Fuh-Mei Duh, Benjamin P. Fairfax, Bahara Saleh, Julian C. Knight, Stephen K. Anderson, Mary Carrington  The American Journal of Human Genetics  Volume 99, Issue 6, Pages 1353-1358 (December 2016) DOI: 10.1016/j.ajhg.2016.09.023 Copyright © 2016 American Society of Human Genetics Terms and Conditions

Figure 1 HLA-C Level Is Associated with rs2395471 in the Promoter Region of HLA-C (A) Association between SNPs located in a 200 kb window around HLA-C and imputed HLA-C level. The Manhattan plot was created with LocusZoom.15 Each dot represents a SNP for which the color depicts the linkage disequilibrium (LD) score (r2) computed with PLINK16 in the European 1KG dataset (n = 228). A complete list of LD scores between rs2395471 and the surrounding 1 Mb SNPs is presented in Table S2. Logistic regression tests for association between the 68,726 SNPs in the HLA locus and HLA-C level was performed in R (version 3.2.4) using the imputed HLA-C level values as a continuous variable. (B) A list of HLA-C lineages that carry either the A or G at the rs2395471 SNP and a map of the HLA-C promoter region are shown. The American Journal of Human Genetics 2016 99, 1353-1358DOI: (10.1016/j.ajhg.2016.09.023) Copyright © 2016 American Society of Human Genetics Terms and Conditions

Figure 2 Association between rs2395471 and HLA-C Cell Surface Level Cell surface level was measured directly in two independent cohorts of (A) 195 African Americans and (B) 174 European Americans. HLA-C cell surface level was measured on CD3+ cells from peripheral blood by flow cytometry. For each plot, the p value from linear regression and R2 (the level of variation explained by the SNP) are provided. The rs2395471 genotypes were determined by Sanger sequencing. The mean with standard deviation is represented. The National Health Institutes office of human subjects research protection approved this study. The American Journal of Human Genetics 2016 99, 1353-1358DOI: (10.1016/j.ajhg.2016.09.023) Copyright © 2016 American Society of Human Genetics Terms and Conditions

Figure 3 The Oct1 Transcription Factor Binds to the Genomic Region Containing rs2395471 Electrophoretic mobility shift assay (EMSA) was performed using nuclear protein extract from HeLa cell line (A and B) and PBMCs (C). Two oligonucleotides were designed, one containing the rs2395471_G allele and one containing the rs2395471_A allele (detailed methods can be found in Li et al.,22 primers are available upon request). (A) The presence of a shift (arrow) indicates that the genomic region containing rs2395471 binds a protein from the HeLa nuclear extract. A supershift is observed when anti-Oct1 antibodies (2 different clones) are added, but not anti-Oct2, anti-Oct3/4, or anti-Sp1 antibodies, indicating that Oct1 is a transcription factor that binds to the rs2395471 genomic region. (B) HeLa nuclear extract binds to oligonucleotides containing rs2395471_A more strongly than it does to rs2395471_G. (C) Nuclear proteins derived from PBMCs of two donors were extracted and incubated with the oligonucleotides and antibodies to anti-Oct1 and anti-Oct3/4. A supershift was detected only in the presence of the anti-Oct1 antibody, confirming the specificity of this TF binding site for Oct1. The American Journal of Human Genetics 2016 99, 1353-1358DOI: (10.1016/j.ajhg.2016.09.023) Copyright © 2016 American Society of Human Genetics Terms and Conditions

Figure 4 Impact of the rs2395471 on the HLA-C Promoter Activity Estimated by Luciferase Assay The HLA-C promoters of two alleles carrying rs2395471_A, C∗01:02 and C∗04:01, and two alleles carrying rs2395471_G, C∗03:04 and C∗08:02, were cloned in a pGL3 plasmid. We mutated this position by site-directed mutagenesis to obtain new plasmids with a G nucleotide at the rs2395471 position for C∗01:02 and C∗04:01 and an A at this position for C∗03:04 and C∗08:02 (detailed methods can be found in Li et al.,22 primers are available upon request). Both HLA-C∗01:02 and C∗04:01 promoters in which rs2395471_A was mutated to rs2395471_G show a significant decrease in promoter activity. Both HLA-C∗03:04 and C∗08:02 promoters in which rs2395471_G was mutated to rs2395471_A show no significant difference in promoter activity. Two-way ANOVA. ∗∗p < 0.01, ∗∗∗∗p < 0.0001. WT: wild-type. The mean of four replicated experiments with standard error is represented. The American Journal of Human Genetics 2016 99, 1353-1358DOI: (10.1016/j.ajhg.2016.09.023) Copyright © 2016 American Society of Human Genetics Terms and Conditions