C-MYC overexpression or growth in serum-containing media induces EMT in HBEC3p53,KRAS. c-MYC overexpression or growth in serum-containing media induces.

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c-MYC overexpression or growth in serum-containing media induces EMT in HBEC3p53,KRAS. c-MYC overexpression or growth in serum-containing media induces EMT in HBEC3p53,KRAS. A, phase contrast photomicrographs showing the morphologic effect observed in HBEC3p53,KRAS (left) following overexpression of c-MYC (middle) or switching from defined serum-free media to serum-containing media (right; ×20 magnification). B, immunoblot for EMT markers in oncogenically manipulated HBEC3 and HBEC17 grown in either KSFM (serum-free) or serum-containing (R10) media. The presence (+) or absence (−) of serum is shown. C, EMT-related genes altered 4-fold or greater in pairwise analysis of HBEC3p53,KRAS and HBEC3p53,KRAS,cMYC comparing cells grown in serum or defined medium (KSFM). Values log2 transformed. *, P < 0.05; **, P < 0.01 (t test). Full-length blots are presented in Supplementary Fig. S8. Mitsuo Sato et al. Mol Cancer Res 2013;11:638-650 ©2013 by American Association for Cancer Research