Activation of multiple RTKs in chondrosarcoma cells.

Slides:

Advertisements

Similar presentations

B A C Figure S1 LEP GAPDH N OA

Advertisements

A novel SHP-1/Grb2–dependent mechanism of negative regulation of cytokine-receptor signaling: contribution of SHP-1 C-terminal tyrosines in cytokine signaling.

Phosphorylation of RelA Ser311 in vivo.

TGF-β1 induces ILK activity in renal tubular epithelial cells.

PGAM5 dephosphorylates ASK1.

HDAC3, but not HDAC2 is in complex with tau protein.

Small-molecule inhibitor QLT-0267 suppresses ILK activity and inhibits its downstream signaling. Small-molecule inhibitor QLT-0267 suppresses ILK activity.

GPR124 promotes cell migration.

PGAM5 associates with and activates ASK1.

Pirh2 promotes p73 ubiquitination in vivo.

Eotaxin induces degranulation and chemotaxis of eosinophils through the activation of ERK2 and p38 mitogen-activated protein kinases by Gita T. Kampen,

The Related Adhesion Focal Tyrosine Kinase (RAFTK) Is Tyrosine Phosphorylated and Participates in Colony-Stimulating Factor-1/Macrophage Colony-Stimulating.

FAS1 domain protein inhibits association of αvβ3 integrin with VEGFR-2 and attenuates VEGF165-induced VEGFR-2 phosphorylation. FAS1 domain protein inhibits.

Supplement Figure 2 HEK 293 cells

Phospho-Twist1–induced TGF-β2 acts as an essential mediator of signaling cross-talk between TGF-βR/Smad2 and PI3K/Akt axes. Phospho-Twist1–induced TGF-β2.

NM-3 induces p21 levels and down-regulation of Cdk2 activity.

Monica Kong-Beltran, Jennifer Stamos, Dineli Wickramasinghe

p38 MAPK activation is required for phosphorylation of Akt at Ser473.

Role of HER2 in mediating acquired resistance to EGFR inhibition.

Leto et al., Supplementary Figure S3

Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE. Immunoblot analysis of apoptotic HCT-8 cell extracts separated by SDS-PAGE.

Silva H Hanissian, Raif S Geha Immunity

Thiocolchicoside inhibits TNF-dependent IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. Thiocolchicoside inhibits.

VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown AZ-521 cells. VacA does not induce phosphorylation of Src in RPTPα constitutive-knockdown.

AMPK directly increases mTORC2 catalytic activity.

Phosphorylation of AMPK-Thr172 before and after metformin treatment in muscle from subjects with type 2 diabetes. Phosphorylation of AMPK-Thr172 before.

eNOS regulated IR-induced NO generation and EGFR signal activation.

co-IP of LEDGF/p75 and MeCP2.

Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis.

Effects of knockdown of DDR1 levels on proliferation and apoptosis.

FOXO3a phosphorylation and expression.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Mapping the Pirh2 and p73 interaction sites.

AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2. AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2.

DNA damage signaling regulates mutant p53 ubiquitination.

Phosphorylation of TET2 by JAK2 in response to hematopoietic cytokines

Down-regulation of the erbB-2 receptor by trastuzumab decreases Akt kinase activation but not MAPK activation. Down-regulation of the erbB-2 receptor by.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Induction of cytotoxic activity in humanized SCC3 tumor-bearing mice treated with anti-PD-1 antibody. Induction of cytotoxic activity in humanized SCC3.

The cross-talk between PARylation and phosphorylation.

TET2 interacts with KLF1 transcription factor.

A, Western blot analysis of vascular endothelial growth factor-C (VEGF-C). A, Western blot analysis of vascular endothelial growth factor-C (VEGF-C). Concentrated.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Expression of dominant-negative RasN17 completely suppresses Ras activation in Rh1 cells. Expression of dominant-negative RasN17 completely suppresses.

ABT-100 inhibits PI3K activity and p85 tyrosine phosphorylation.

SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.

IGF-II antibodies inhibited IGF-II-induced phosphorylation of IGF-IR and insulin receptor (IR) in the breast cancer MCF-7 cells. IGF-II antibodies inhibited.

Changes in signal transduction pathway induced by gefitinib.

Characterization of monoclonal antibody 4

WP1066 induces caspase-dependent apoptosis.

The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. Serum-starved.

Osteoactivin is overexpressed in aggressively bone metastatic 4T1 subpopulations versus weakly bone metastatic breast cancer populations. Osteoactivin.

AR is transnitrosylated by HSP90.

ODC protein expression in paired benign and cancer tissue obtained from patients with PCA. The protein expression was measured by immunoblot analysis in.

A, effect of Z-VAD treatment on synchronized HeLa MR cells.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

SAHA blocks IR-induced increase of RAD51 protein in MM cells.

A, TSG-6 staining in human prostate.

BCR/ABL expression, tyrosine phosphorylation, and signaling in dasatinib- and imatinib-resistant cell lines and the ubiquitin inhibitor lactacystin modifies.

EGCG affects growth factor receptor signaling in H2111, H358, and H460 NSCLC cells. EGCG affects growth factor receptor signaling in H2111, H358, and H460.

Effect of bevacizumab on the proliferation of A2780 cells.

Induction of PARP cleavage (A) and activation of caspases (B) after treatment with a combination of TRAIL and cisplatin. Induction of PARP cleavage (A)

Expression and induction of HER2 and HPSE in 231BMBC cells.

Effect of MIF siRNA on the production of MMP-13 induced by LPA

Posttranslational phosphorylation of p53 by platinum drugs in ovarian tumor cells. Posttranslational phosphorylation of p53 by platinum drugs in ovarian.

In the presence of human monocytes, estradiol increased breast cancer cell dissemination to the periphery via CCL2 and CCL5. In the presence of human monocytes,

Association of TCTP with Pim-3 in human pancreatic cancer cell lines.

P38 activation requires a d-GM3–dependent membrane molecular complex of uPAR, caveolin-1, and integrin α5β1. p38 activation requires a d-GM3–dependent.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

D-GM3 promotes uPAR clustering on the cell surface and activates p38 MAPK. A, uPAR expression in cells was either knocked down by treatment with 4 independent.

Presentation transcript:

Activation of multiple RTKs in chondrosarcoma cells. Activation of multiple RTKs in chondrosarcoma cells. A, the phosphorylation status of 42 RTKs in chondrosarcoma cells under serum-depleted conditions was assessed by phospho-RTK arrays. Anti-RTK antibodies spotted on nitrocellulose membrane in duplicate were incubated with cell lysates followed by secondary anti-phosphotyrosine antibody detection. Positive controls are represented in the 4 corners of each blot; negative antibody isotype controls and PBS control are represented in E13-20 and E21-22, respectively. The location and names of capture antibodies are listed in the first and second column of B. B, qualitative heat-blot map of RTK array data. +++, RTK signal is higher than positive controls; ++, signal is similar to positive controls; +, signal is lower than positive control but 5-fold higher than antibody isotype negative controls; −, low signal. C, validation of phospho-RTK array data by immunoblot (IB) or immunoprecipitation (IP)/IB analysis. Yi-Xiang Zhang et al. Clin Cancer Res 2013;19:3796-3807 ©2013 by American Association for Cancer Research