Adhesion to fibronectin protects Mcl-1 knockdown cells from apoptosis.

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Adhesion to fibronectin protects Mcl-1 knockdown cells from apoptosis. Adhesion to fibronectin protects Mcl-1 knockdown cells from apoptosis. A. Doxycycline-treated WM793TR-Ctl shRNA and WM793TR-B-RAF shRNA cells were transfected with either control or Mcl-1 siRNAs. Following transfection and serum starvation, WM793 cells were replated onto agar or fibronectin-coated dishes for 48 h in serum-free medium. Harvested cells were analyzed for cleaved caspase-3 staining by flow cytometry. B. Quantitation of cleaved caspase-3 staining from three independent experiments. Karen Boisvert-Adamo et al. Mol Cancer Res 2009;7:549-556 ©2009 by American Association for Cancer Research