Knockdown of ERBB3, NRG1, or ERBB2 in H358-TwistER cells reduces basal PI3K–AKT signaling and ablates serum-independent proliferation before EMT. A and.

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Knockdown of ERBB3, NRG1, or ERBB2 in H358-TwistER cells reduces basal PI3K–AKT signaling and ablates serum-independent proliferation before EMT. A and B, lysates from H358-TwistER cells before EMT 72 hours after transfection with indicated siRNA oligos, se... Knockdown of ERBB3, NRG1, or ERBB2 in H358-TwistER cells reduces basal PI3K–AKT signaling and ablates serum-independent proliferation before EMT. A and B, lysates from H358-TwistER cells before EMT 72 hours after transfection with indicated siRNA oligos, serum-starved overnight. C, immunoprecipitation of indicated proteins from H358-TwistER lysates 72 hours after transfection with indicated oligos, serum-starved overnight. D, proliferation of H358-TwistER cells before EMT in the absence of serum after transfection with indicated siRNAs. IP, immunoprecipitation; WCL, whole-cell lysates; IgG, immunoglobulin G; NT, untreated. Megan B. Salt et al. Cancer Discovery 2014;4:186-199 ©2014 by American Association for Cancer Research