Keratinocyte-specific disruption of the Stat3 gene by the Cre-LoxP system. Keratinocyte-specific disruption of the Stat3 gene by the Cre-LoxP system. A,

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Keratinocyte-specific disruption of the Stat3 gene by the Cre-LoxP system. Keratinocyte-specific disruption of the Stat3 gene by the Cre-LoxP system. A, a partial genomic map of the Stat3 gene before and after Cre-mediated disruption. The exons to be excised (16–21, solid boxes) were flanked by two identically oriented LoxP sites (dotted triangles). The three primers indicated (A, B, and C) were used for the detection of wt, floxed (LoxP), and disrupted (null) Stat3 alleles, respectively. B, allele-specific PCR analysis performed on tail (T) and keratinocyte (KC) genomic DNA preparations; the primers used are described in Materials and Methods (M; Quick-Load 100-bp DNA ladder). C, analysis of Stat3 protein levels in epidermal lysates (30 μg) pooled from three animals for each genotype. β-Actin was used to control for equal loading. Marco De Andrea et al. Cancer Res 2010;70:7938-7948 ©2010 by American Association for Cancer Research