Fig. 4 Enzyme activity of PE-ProK/Pt tuned by changing temperature or NIR light. Enzyme activity of PE-ProK/Pt tuned by changing temperature or NIR light.

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Fig. 4 Enzyme activity of PE-ProK/Pt tuned by changing temperature or NIR light. Enzyme activity of PE-ProK/Pt tuned by changing temperature or NIR light. (A) Schematic illustrates PE-ProK/Pt catalyzes proteolysis upon NIR irradiation. (B and C) Relative fold increase of PE-ProK (B) and PE-ProK/Pt (C) activities by heating in water bath (45°C) or exposure to NIR light (3.8 W cm−2, 20 min). ***P < 0.001 analyzed by Student’s t test (n = 3). (D) SDS-PAGE of casein after different treatments (bottom). Casein is incubated with PE-ProK/Pt or ProK, and NIR irradiation (3.8 W cm−2, 20 min) is applied to turn on the activity of PE-ProK/Pt. The remaining casein is stained by Coomassie Brilliant Blue G250 (top). M means protein marker (from top to bottom: 250, 150, 100, 70, 50, 35, and 25 kDa). (E) PE-ProK/Pt catalyzes the digestion of phycocyanin, a red fluorescent protein. The alginate-Ca2+ hydrogel containing PE-ProK/Pt and phycocyanin is exposed to NIR light (2.5 W cm−2) for 30 min. The dashed line represents the border of laser irradiation region. (Photo credit: Song Zhang, East China Normal University.)‏ Song Zhang et al. Sci Adv 2019;5:eaaw4252 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).