Untargeted LC/MS metabolite profiling of DFMO-treated HT-29 colorectal cancer cells. Untargeted LC/MS metabolite profiling of DFMO-treated HT-29 colorectal.

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Untargeted LC/MS metabolite profiling of DFMO-treated HT-29 colorectal cancer cells. Untargeted LC/MS metabolite profiling of DFMO-treated HT-29 colorectal cancer cells. Metabolite profiling identified differentially expressed metabolites in DFMO-treated (300 nmol/L) compared with untreated HT-29 cells (P < 0.05). A, plot of retention time versus mass for 1,350 aligned features detected by untargeted molecular feature extraction in all four replicates from at least one treatment group. B, PCA showing that the DFMO-treated group clustered separately from the untreated HT-29 cell group along the principal component 1 (PC1) axis, which represents 67% of total variance. C, heatmap representation of unsupervised hierarchical clustering analysis of data from A. Relative expression of each metabolite is represented by a color code where red represents greatest abundance and blue represents lowest abundance, relative to mean values for the 1,350 denoted metabolites. D, volcano plot analysis indicates that 596 metabolites were altered in expression twofold or more after DFMO treatment (*, P < 0.05). Mavee Witherspoon et al. Cancer Discovery 2013;3:1072-1081 ©2013 by American Association for Cancer Research