Effect of SPINDLIN1 protein on cancer cell proliferation and invasion.

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Cell Physiol Biochem 2013;32: DOI: /

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Effect of SPINDLIN1 protein on cancer cell proliferation and invasion. Effect of SPINDLIN1 protein on cancer cell proliferation and invasion. A, four clones of HeLa cells stably expressing pBPLV-SPINDLIN1 and 2 clones of control cells were used in colony-forming assays. The expression of C-MYC-SPINDLIN1 in each clone was analyzed by Western blotting. B, five hundred cells per well were plated in 6-well plates and incubated for 2 to 3 weeks. Colonies were stained with crystal violet (left), and the numbers of colonies are shown (right). SC1, N = 3, *, P < 0.05 compared with CC1 (Student t test). SC2, SC3, N = 3, ***, P < 0.001 compared with CC1 (Student t test). SC, SPINDLIN1 clone; CC, control clone. C, a total of 3 × 106 HeLa cells expressing SPINDLIN1 (SC1–SC4) or control cells (CC1 and CC2) were injected into one flank of each of 4 nu/nu mice. One month after injection, the mice were sacrificed and tumors were removed (top left). The tumor weight (bottom left) and the tumor volume (right) are shown as the means ± SD. N = 16; **, P < 0.01 compared with control (Student t test). D, HeLa and A549 cells overexpressing C-MYC-SPINDLIN1 or C-MYC (control) were used for the in vitro invasion assay. The cells that migrated through the Matrigel after 36 hours were stained with crystal violet and are shown at randomly selected fields (left and middle). The number of cells is expressed as the means ± SD (right). N = 3; ***, P < 0.001 compared with control (Student t test). Jing-Xue Wang et al. Mol Cancer Res 2012;10:326-335 ©2012 by American Association for Cancer Research