Effects of UVB on AKT in SKH-1 mouse epidermis.

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Effects of UVB on AKT in SKH-1 mouse epidermis. Effects of UVB on AKT in SKH-1 mouse epidermis. Mice were UVB irradiated (6 kJ/m2) and epidermal protein was harvested at various time points and resolved by SDS-PAGE. Blots were probed with antibodies specific for phosphorylated AKT (Ser473) and total AKT. Western blots for phosphorylated AKT were normalized to total levels of AKT by densitometric analysis (A). *, P < 0.05, Student's t test. Activation of UVB-induced AKT (Ser473) is shown in individual mice at 0.5 and 1 hour postirradiation (B). Michael A. Bachelor et al. Mol Cancer Res 2005;3:90-99 ©2005 by American Association for Cancer Research