Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative. Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative.

Slides:

Advertisements

Similar presentations

Volume 44, Issue 3, Pages (March 2016)

Advertisements

Plasmacytoid dendritic cells efficiently cross-prime naive T cells in vivo after TLR activation by Juliette Mouriès, Gabriel Moron, Géraldine Schlecht,

Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Uptake of T-MPs for DC maturation and antigen presentation.

Interaction between B7-H1 and PD-1 determines initiation and reversal of T-cell anergy by Fumihiko Tsushima, Sheng Yao, Tahiro Shin, Andrew Flies, Sarah.

Volume 44, Issue 3, Pages (March 2016)

Volume 40, Issue 2, Pages (February 2014)

IRF4 is required for anabolic induction of CD4+ T cells.

B-cell differentiation associates with down-regulation of vimentin expression. B-cell differentiation associates with down-regulation of vimentin expression.

Volume 1, Issue 3, Pages (March 2012)

Fig. 8. mRIPO elicits neutrophil influx followed by DC and T cell infiltration into tumors. mRIPO elicits neutrophil influx followed by DC and T cell infiltration.

Volume 40, Issue 3, Pages (March 2014)

IL-6 is dispensable for the suppressive activity of MDSC on primary CD4+ T-cell activation. IL-6 is dispensable for the suppressive activity of MDSC on.

Smaller T cell zone FRC areas in aged spleens.

Functional interaction of Eμ-Tcl1 tumor cells with FDC networks.

Volume 44, Issue 3, Pages (March 2016)

Maturation, Ag presentation capacity, and IL-12 production of DCs

Sialylated Ab-ICs promote enhanced humoral immune responses.

VLPs activate DCs and antigen-specific CD8+ T cells via the STING and cGAS pathway. VLPs activate DCs and antigen-specific CD8+ T cells via the STING and.

MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific T-bet+ population. MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific.

Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient mice. Enhanced proliferation and cytokine production in DGKζ and Cbl-b deficient.

Volume 45, Issue 2, Pages (August 2016)

Altered cytokine production by Klrk1−/− NOD CTL

Viperin−/−mice have an increased IFNγ Th1 response.

Confirmation of novel RSV CD8+ T cell epitopes.

Volume 25, Issue 1, Pages (January 2017)

Antisense Targeting of cFLIP Sensitizes Activated T Cells to Undergo Apoptosis and Desensitizes Responses to Contact Dermatitis Dan V. Mourich, Jessica.

Volume 45, Issue 1, Pages (July 2016)

Volume 44, Issue 3, Pages (March 2016)

Volume 33, Issue 6, Pages (December 2010)

Volume 13, Issue 12, Pages (December 2015)

IFN-γ induces TNF family ligand protein expression in vitro and in vivo. IFN-γ induces TNF family ligand protein expression in vitro and in vivo. (A and.

Volume 29, Issue 6, Pages (December 2008)

Katelyn T. Byrne, Robert H. Vonderheide Cell Reports

Volume 135, Issue 3, Pages (September 2008)

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

Peptide concentration controls digital NFAT1 nuclear translocation kinetics in a responder fraction of stimulated naive OT-I cells. Peptide concentration.

Volume 17, Issue 2, Pages (February 2009)

T Cells with Low Avidity for a Tissue-Restricted Antigen Routinely Evade Central and Peripheral Tolerance and Cause Autoimmunity Dietmar Zehn, Michael.

Volume 20, Issue 11, Pages (September 2017)

Volume 19, Issue 9, Pages (May 2017)

Volume 33, Issue 4, Pages (October 2010)

Volume 32, Issue 1, Pages (January 2010)

Volume 15, Issue 11, Pages (June 2016)

Ag 85A–specific immune responses.

Volume 38, Issue 2, Pages (February 2013)

APCs from hypertensive mice present antigens more efficiently.

Socs1 KD tumors exhibit a more T cell–inflamed phenotype and increased CD8+ T cell activation. Socs1 KD tumors exhibit a more T cell–inflamed phenotype.

IL-33, IL-25, and TSLP induce a distinct phenotypic and activation profile in human type 2 innate lymphoid cells by Ana Camelo, Guglielmo Rosignoli, Yoichiro.

Volume 20, Issue 3, Pages (March 2012)

Volume 25, Issue 4, Pages (April 2017)

Members of IL-1 family of cytokines favor the generation of IL-3–secreting CD4+ T cells in vitro. Members of IL-1 family of cytokines favor the generation.

CD8 T cell memory alters the immune profile in enhanced HLH without altering viral load. CD8 T cell memory alters the immune profile in enhanced HLH without.

Characteristics of GM-CSF–secreting CD4+ T cells.

RvD1 increased anti-inflammatory macrophages and Treg cells in the sciatic nerves of EAN rats. RvD1 increased anti-inflammatory macrophages and Treg cells.

Vaginal CD11c+ DCs from IL-17A−/− mice are impaired in potentiating Th17 responses because of diminished IL-1β production. Vaginal CD11c+ DCs from IL-17A−/−

GC reaction is impaired in NOTCH2 knock-in mice.

Stereotactic radiotherapy increases Tregs in tumors.

pDCs from the melanoma environment responded to TLR-L stimulation.

Volume 13, Issue 11, Pages (December 2015)

MDSC-derived IL-6 enhances tumor progression through the inhibition of tumor-specific TH1 development and of their helper activity for CD8+ T cells. MDSC-derived.

Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation. Hypoxia upregulates CD137 expression in T lymphocytes undergoing activation.

The effect of βAR signaling on the generation of a cytotoxic CD8+ T-cell response in vivo. The effect of βAR signaling on the generation of a cytotoxic.

Mice with a B cell–specific deletion of Ets1 do not have increased CD4+ T cell activation. Mice with a B cell–specific deletion of Ets1 do not have increased.

IL2Cx alone or in combination with anti–CTLA-4 increases the CD8/Treg ratio in the tumor. IL2Cx alone or in combination with anti–CTLA-4 increases the.

Volume 25, Issue 4, Pages (April 2017)

Fig. 3 MCA-mediated neutrophil recruitment accelerates bacterial clearance in the skin. MCA-mediated neutrophil recruitment accelerates bacterial clearance.

Immunization with IR or F/T elicits activated OT-I cells of distinct phenotypes. Immunization with IR or F/T elicits activated OT-I cells of distinct phenotypes.

Varying the MHC-I affinity, TCR affinity or antigen dose alters the phenotype of CD8 T cells ex vivo. Varying the MHC-I affinity, TCR affinity or antigen.

IL-17 produced by innate sources in the FGT is critical for potentiating Th17 responses primed by vaginal APCs. Vaginal cells from hormone cycle–matched.

Presentation transcript:

Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative. Hypoxic Ag-specific CD8+ T cells are less functional and less proliferative. Effect of hypoxia on CD8+ T cells. (A) Quantification of CD8+ T cell distribution in hypoxic (ELK-Cy3+) or normoxic (ELK-Cy3−) Colon38 tumor samples. Fluorescent immunohistochemistry images from tumor-bearing mice (day 11 after tumor inoculation) were labeled with anti-CD8 and ELK-Cy3 Abs. Using these images, CD8+ T cell densities were calculated. Each dot represents one tumor section; three to eight sections were analyzed per tumor (one tumor per mouse). Circles and squares represent CD8+ T cell density in normoxic (n = 17) areas and hypoxic areas (n = 8), respectively. Error bars depict ±SEM. Differences between means were analyzed using an unpaired t test. p = 0.2793. (B) A total of 5 × 106 OT-1 splenic and lymph node cells were stimulated with SIINFEKL at a concentration of 12.5 μg/ml for 24 h. Following antigenic stimulation, cells were incubated under hypoxic (0.5% O2) or normoxic (21% O2) conditions for another 24 h. Cells were surface-stained for CD45 and CD8 and fixed, and intracellular cytokine IFN-γ was analyzed using flow cytometry. Representative dot plot images (left). Bar graphs (right) represent an average of three experiments (n = 3). Error bars depict ±SEM. Differences between means were analyzed using a Mann–Whitney U test. **p < 0.01. (C) Cell Trace Violet (CTV)–labeled cells were stimulated with OVA peptide fragment (SIINFEKL) at a concentration of 12.5 μg/ml for 24 h. Following antigenic stimulation, OT-1 cells were incubated under hypoxic (0.5% O2) or normoxic (21% O2) conditions for another 24 h. Cells were surface-stained for CD45 and CD8 and fixed, and CTV fluorescence was analyzed using flow cytometry. Representative histograms from one of three similar experiments. (D) A total of 5 × 106 OT-1 splenic and lymph node cells were stimulated with SIINFEKL at a concentration of 12.5 μg/ml for 24 h. Following antigenic stimulation, cells were incubated under hypoxic (0.5% O2) or normoxic (21% O2) conditions for 0, 16, 26, and 44 h. One set of hypoxic (26 h) cells was reoxygenated at 16 h, and the culture continued under normoxic (21% O2) conditions. Cells were surface-stained for CD45 and CD8 and fixed, and intracellular cytokine IFN-γ was analyzed using flow cytometry. Line graphs represent an average of three experiments (n = 3). Error bars depict ±SEM. Means were compared using ordinary two-way ANOVA. *p < 0.05, **p < 0.01. Aditi Murthy et al. ImmunoHorizons 2019;3:149-160 Copyright © 2019 The Authors