RLM-RACE mapping the 5' end of BORIS mRNA.

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RLM-RACE mapping the 5' end of BORIS mRNA. RLM-RACE mapping the 5' end of BORIS mRNA. (A) BORIS transcriptional start site in testis (T), DNMT1-/-, 3b-/- HCT116 cells (D), and epithelial ovarian cancer (EOC) sample #7 (E). Superscripted numbers indicate the number of sequenced clones displaying the indicated 5' end (first three nucleotides underlined). The right arrow shows the NCBI-predicted transcriptional start site. The vertical line indicates the exon 1-intron 1 boundary as mapped by RLM-RACE. Asterisks indicate the transcriptional start site of BORIS in testis reported in (1). (B) Diagram of the 5' region of BORIS. The right arrow displays the NCBI-predicted transcriptional start site, and the filled region in exon 2 shows the start of the open reading frame. EMBOSS CpG plot (26) identified a CpG island (225 bp, 22 CpGs, 73% GC, observed/expected CpG ratio = 0.73) overlapping the transcriptional start site. The regions analyzed by sodium bisulfite sequencing and pyrosequencing, along with the number of CpGs contained within each region, are shown. Anna Woloszynska-Read et al. Cancer Immun 2007;7:21 Copyright © 2007 by Adam R. Karpf