1 OPCML negatively regulates a specific repertoire of RTKs

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1 OPCML negatively regulates a specific repertoire of RTKs 1 OPCML negatively regulates a specific repertoire of RTKs. Western blots demonstrating that OPCML negatively regulates EPHA2, FGFR1, FGFR3, HER2, and HER4 but not EPHA10, FGFR2, EGFR, HER3, VEGFR1, and VEGFR3; shown in stably transfected SKOV-3 cells (A), ... 1 OPCML negatively regulates a specific repertoire of RTKs. Western blots demonstrating that OPCML negatively regulates EPHA2, FGFR1, FGFR3, HER2, and HER4 but not EPHA10, FGFR2, EGFR, HER3, VEGFR1, and VEGFR3; shown in stably transfected SKOV-3 cells (A), containing pcDNA3.1 vector only (–) and pcDNA3.1-OPCML with lower (SKOBS-3.5) and higher (BKS2.1) OPCML expression; polyclonal transiently transfected PEO1 cells (B) with vector only (–) and OPCML (+). OSE-C2 (normal ovarian surface epithelial) cells (C), physiologically expressing OPCML which were untreated (–), or transiently transfected with nonsilencing duplex (Non-si), or OPCML-directed siRNA duplexes (si) demonstrated reciprocal upregulation of the same specific repertoire of RTKs. β-tubulin control for loading is shown in bottom panel. D, immunofluorescence microscopy showing HER2 and EPHA2 expression in vector control SKOBS-V1.2 and stable transfection OPCML expressing BKS2.1 cells. Abundant HER2 and EPHA2 expression seen in SKOBS-V1.2 (green) is abrogated in BKS2.1 associated with expression of OPCML (red). Scale bars, 10 μm. E, comparison of vector (white bars) and OPCML (black bars) transfected SKOV-3 cells showing quantification of the Immunofluorescent pixel intensity for OPCML, FGFR1, EPHA2, HER2, EGFR, and FGFR2 demonstrates the same OPCML-associated RTK spectrum of downregulation by a second method. F, a 144-hour in vitro growth assay compares vector control SKOBS V1.2 (OPCML deficient), BKS2.1 (strongly expressing OPCML), SKOBS-3.5 (lower OPCML expressor), and OSE-C2 (normal ovarian surface epithelial cell line). Cell proliferation was quantified by MTT assay showing OPCML expression is strongly growth suppressive. Arthur B. McKie et al. Cancer Discovery 2012;2:156-171 ©2012 by American Association for Cancer Research