DNase and proteinase K treatment of WT R2866 and R2866ΔampG gene deletion mutant biofilms. DNase and proteinase K treatment of WT R2866 and R2866ΔampG.

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DNase and proteinase K treatment of WT R2866 and R2866ΔampG gene deletion mutant biofilms. DNase and proteinase K treatment of WT R2866 and R2866ΔampG gene deletion mutant biofilms. (A) Overnight growth determined by measuring OD620. (B) Biofilm formation by WT R2866 and the R2866ΔampG gene deletion mutant determined by measuring crystal violet light A560 in the presence of proteinase K (100 μg/ml) or DNase (100 μg/ml). (C) Relative DNA abundance in the R2866ΔampG gene deletion mutant compared to that in WT R2866 determined by qPCR detection of the Haemophilus protein D gene (hpd). One-way analysis of variance with Tukey’s multiple-comparison test was used for statistical analysis (NS, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001). Sara Marti et al. mSphere 2017; doi:10.1128/mSphere.00329-16