Real-time PCR with SYBR Green Master Mix was used to quantify the expression levels of 84 genes ontologically related and regulated by TGF-β /BMP signaling.

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Real-time PCR with SYBR Green Master Mix was used to quantify the expression levels of 84 genes ontologically related and regulated by TGF-β /BMP signaling pathway or the 84 genes of the signal transduction pathway finder arrays (SA Biosciences). Real-time PCR with SYBR Green Master Mix was used to quantify the expression levels of 84 genes ontologically related and regulated by TGF-β /BMP signaling pathway or the 84 genes of the signal transduction pathway finder arrays (SA Biosciences). The heat map shows absolute mRNA copy numbers, which were calculated from PCR cycle thresholds (Ct). For example, on the color-coded log2 scale, a value of 10 represents 210 or 1,024 transcripts. Two endogenous controls, GAPDH and ACTB, were used for normalization. Right, functional gene clustering (with major groups according to the array manual). Nandor Garamszegi et al. Mol Cancer Res 2009;7:319-329 ©2009 by American Association for Cancer Research