Structure and Function The Cell Membrane Structure and Function

Agenda: 5/6 Grab a paper from the basket! Order of Operations: Egg Lab / Types of Solutions mini poster Finish test (in computer lab) Cell Transport Notes (from website) Time for vocab project Reminders: Mock EOC on Thursday, 3rd section of vocab project due 5/24!

Cell Membrane Functions Cell membrane surrounds the cell, regulating the transport of materials into and out of the cell

Cell Membrane Structure Lipid Bilayer: a double layer of phospholipids that make up the cell membrane

Phospholipid Phosphate Head: Two Fatty Acid Tails: Two Layers Polar Hydrophilic (attracted to water) Two Fatty Acid Tails: Nonpolar Hydrophobic (avoids water) Two Layers Outsides: Polar Heads Inside: Nonpolar Tails

Other lipids ? Lipids Fatty acids Fats and waxes Steroids Cholesterol Testosterone

Cell Membrane Structure Phospholipids are arranged so hydrophilic (water loving) ends face outside and hydrophobic (water fearing) tails create the interior

Cell Membrane Structure Phospholipid Bilayer Transmembrane Proteins Cholesterol (a steroid) Glycoprotein and Carbohydrate

Cell Membrane Structure Transmembrane Proteins Job: Attachment point between cells Anchor cell in place Create channels for large and/or polar molecules to pass

Cell Membrane Structure Cholesterol Job: Maintains fluidity of cell membrane

Cell Membrane Structure Glycolipids and Carbohydrates Job: Serve as cell markers for identification

Cell Membrane Structure The Fluid Mosaic Model The membrane is dynamic – molecules are constantly moving

Selectively Permeable The cell membrane is selectively permeable, meaning some substances can pass through the cell membrane, but others can’t! “Permeable” is the ability for something to pass through

You are working on one or the other! EGG LAB INSTRUCTIONS You are working on one or the other! Strain egg over sink Rinse egg and cup Make observations about egg on lab paper Place egg back in cup and pour syrup over the egg Replace plastic wrap and place cup on back counter Wipe tables down with Clorox wipes and WASH YOUR HANDS! TYPES OF SOLUTIONS For each type of solution, WRITE THE TERM, DEFINE THE TERM, and then draw the ANIMAL CELL and the PLANT CELL on page 186 in the GREEN BIO textbook. Make sure to label the direction(s) that water is moving!

Passive Transport Osmosis and Diffusion Raisin demonstration needs to be set up first to allow time for osmosis to take place.

Selectively Permeable The cell may transport substances Passive Transport: No Energy required Active Transport: Uses energy (ATP)

Passive Transport Movement of a substance into/out of a cell without using energy HIGH  LOW concentration 3 Types Simple Diffusion Facilitated Diffusion Osmosis

About Concentration… We just learned that substances move from HIGHLOW concentration in passive transport…but what do we mean by concentration? Means amount dissolved in a container How do you like your sweet tea? a. Unsweet (low concentration of sugar) b. Sweet c. Super sweet(high concentration of sugar) Solute = Substance dissolved in water The sugar is dissolved at different concentrations. Unsweet tea has a low concentration of sugar, and super sweet tea has a high concentration of sugar.

Concentration Gradients A concentration gradient is the difference in the concentration, or amount, of something in a space In the box below, the dots have a higher concentration on the left than the right, so they begin movement to the right When the concentration gradient is the same throughout the area, it is in a dynamic equilibrium Particles are moving from one side to the other, but at the same rate. After this slide, lead into the food coloring discussion. Have them write down observations after they add food coloring to water. Then do diffusion slide and ask them to observe the water again. They should be able to reach the conclusion that the food coloring is diffusing to create equilibrium. The students can stir the water to help equilibrium be reached faster. Picture: www.hartnell.edu

Passive Transport: Diffusion Diffusion is when a substance moves from an area of high concentration to low concentration area Diffusion will continue until balanced/equal “at equilibrium”

Facilitated Diffusion Diffusion of large molecules through special protein channels Ex. Ions, glucose Still happens without energy being used!

Passive Transport: Osmosis Osmosis is the diffusion of water across a selectively permeable membrane Water moves from an area of high concentration (lots of water) to low concentration (little water) Passive Transport: Water moves with its concentration gradient No energy is required Picture: scienceaid.co.uk

About Osmosis Water can move into or out of the cell, depending on the environment (solution) that the cell is in Solvent + Solute = Solution Solvent does the dissolving (ex. Water) Solute substance dissolved (ex. Salt)

EQUAL amounts of solute in/out of cell. Describing Solutions Isotonic Solution “same or equal” Hypertonic Solution “above/over/higher” Hypotonic Solution “below/under/lower” EQUAL amounts of solute in/out of cell. HIGHER solute outside. LOWER solute outside.

How Osmosis Works Isotonic Hypertonic Hypotonic “same or equal” “above/over/higher” Hypotonic “below/under/lower” Water in/out. Plant Cells Flaccid, Central Vac. not full Animal Cell** No Change to cell Water out Plasmolysis, Cell death Animal Cell Dehydration, Cell shrivels Water in. Plant Cells** Turgid, Central Vac. Full Lysed, Cell bursts

Osmosis Onion in Salt Water Blood Cells Same Swelling Shrinking If time permitting, discuss the pH of blood at 7.3 norm. The blood cells have a buffer that tries to regulate the cell at 7.3. If something happens that makes it too acidic or basic, the buffers can fail and cause problems. In the case of the fish, the pH became to high in the water for them to handle. Diffusion caused water to swell and rupture their cells, thus causing them to die. Fish generally have higher solute concentration inside their bodies, and the drastic change in pH alters this and affects them negatively. Picture: www.peoi.org

Check for understanding After an ice storm, road crews salt the roadways to melt ice and increase traction for cars. What happens to the plants on the side of the road? Explain why.

Isotonic, Hypotonic, or Hypertonic? 10% NaCL Environment 90 % H2O CELL 10% NaCL 90 % H2O

Water Goes? Isotonic-Water goes in/out (balanced); cell stays the same! 10% NaCL Environment 90 % H2O CELL 10% NaCL 90 % H2O

Isotonic, Hypotonic, or Hypertonic? 10% NaCL Environment 90 % H2O CELL 20% NaCL 80 % H2O

Water Goes? Hypotonic-water goes IN! Cell swells! 10% NaCL Environment 90 % H2O CELL 20% NaCL 80 % H2O

Isotonic, Hypotonic, or Hypertonic? 10% NaCL Environment CELL 5% NaCL

Water Goes? Hypertonic-water goes OUT-Cell Shrinks!! 10% NaCL Environment Often, you will just be given the solute concentration in the environment. What is the water concentration? Would it move in or out? CELL 5% NaCL

Movement Requiring Energy Active Transport Movement Requiring Energy

Active Transport Molecules move across the membrane AGAINST their concentration gradient Moving LOW to HIGH ENERGY is required! (imagine trying to push your way through a crowd vs. going with the flow…) Two Types Protein Pumps Bulk Transport

Protein Pumps Transport small molecules/ions AGAINST their concentration gradients (LOW HIGH) Uses ATP (cell energy) Notice the ATP

Bulk Transport Larger molecules (proteins, starch) are transported by vesicles that merge with the cell membrane Exocytosis-contents leaving the cell through the membrane Endocytosis-contents entering in vesicles (pinocytosis and phagocytosis)

Finish up egg lab! Strain egg liquid over sink Throw away cup in big trash can Place egg in a bowl and take it back to your seat! Finish lab observations /definitions / conclusion / questions and turn in! When finished with egg, throw it away in big trash can!

Egg Lab Procedure Pour liquid through strainer over sink Pour as little of the syrup into the sink as possible! Throw away cup with syrup (into big trash can) Label a new cup with names of group members Make observations about egg and place in new cup Pour distilled water gently over the egg and place plastic wrap back over cup While you are waiting for a sink: Work on Cells Review Notes and Labeling Work on Types of Solutions assignment from yesterday

Homeostasis Lab Groups 2-3 (NO MORE THAN 3!) One person will volunteer to do jumping jacks and have vitals measured a certain points in the experiment. Heart rate Breathing rate Perspiration level Record data, answer lab questions with partner(s) Graph if we have time???

Homeostasis Maintaining Balance

Homeostasis The process by which an organism’s internal environment is kept in equilibrium (stable) in spite of changes in the external environment Examples: Internal Temperature Sweating removes excess body heat Shivering increases heat production Water balance Kidneys adjust water amount in urine A physical response to stress Breathing/heart rate increases Pupils dilate Sweating)

Homeostasis Homeostasis requires energy For active transport to deliver needed molecules T0 rid cell of toxins To move to avoid danger or find food

Homeostasis in Cells Cells can maintain homeostasis in a number of ways… Controlling substances that cross the membrane Active and Passive transport)

Homeostasis in Cells Cells can maintain homeostasis in a number of ways… Buffers Cells produce buffers to help maintain a constant pH. Examples: Human blood cells must maintain a pH of 7.35-7.45 Cells in stomach maintain a pH of 3 Cells in Small Intestine maintain a pH of 6 What is a buffer? A chemical that can raise or lower the pH (it can behave like an acid or a base)

Yolk

Egg in Syrup Layer of water over the syrup

Poured egg into strainer Water / syrup into trash Notice wrinkled appearance of egg

Notice decreased size of egg Syrup

Egg in water

Homeostasis Maintenance of homeostasis is vital for life! https://www.youtube.com/watch?v=6fhbbFd4icY

Osmosis and Diffusion https://www.youtube.com/watch?v=IaZ8MtF3C6M

Cells and Cell Transport Test Review!

Which cellular process is most closely related the presence of chloroplasts in eukaryotes? Metabolism Photosynthesis Aerobic respiration Lactic acid fermentation

Which of the following serves as the cell’s boundary from its environment? Mitochondria Cell membrane Chloroplast Channel protein

Where would you need more… Mitochondria? Chloroplasts? Cell organelles can be more concentrated based on needs!

Which of the following is a function of the cell membrane? Breaks down lipids, carbohydrates, and proteins in the body Stores water, salt, proteins, and carbohydrates Keeps the cell wall in place Regulates which materials enter and leave the cell

The cell membrane contains channels and pumps that help move materials from one side to the other. What are these channels and pumps made of? Carbohydrates Lipids Bilipids Proteins

Diffusion is the movement of molecules from: An area of low concentration to an area of high concentration An area of high concentration to an area of low concentration An area of equilibrium to an area of high concentration All of the above

Diffusion occurs because: Molecules constantly move and collide with each other The concentration of a solution is never the same throughout a solution The concentration of a solution is always the same throughout a solution Molecules never move or collide with each other

When the concentration of molecules on both sides of the membrane is the same, the molecules will: Move across the membrane to the outside of the cell Stop moving across the membrane Move across the membrane in both directions Move across the membrane to the inside of the cell

The diffusion of water across a selectively permeable membrane is called: Osmotic pressure Osmosis Facilitated diffusion Active transport

An animal cell that is surrounded by fresh water will burst because the osmotic pressure causes: Water to move into the cell Water to move out of the cell Solutes to move into the cell Solutes to move out of the cell

Which means of particle transport requires input of energy from the cell? Diffusion Osmosis Facilitated Diffusion Active Transport

BioFacts 3.2 2/26 2. Energy released by the cells mitochondrion is stored as A. ATP B. glucose C. DNA 3. A cell’s volume grows faster than its surface area, so if a cell gets too large… A. its surface area to volume ratio will decrease B. its outer surface will harden like an eggshell C. it will not be able to take in nutrients or expel waste 4. List the levels of organization from the simplest to the most complex. 5. List FOUR elements that ALL cells have in common.

BioFacts 3.3 2/28 6. Explain selectively permeable. 7. Explain Fluid Mosaic Model. 8. ___ ___ create channels for large and/or nonpolar substances to pass through the membrane. 9. Draw a label a phospholipid. 10. Define Homeostasis. (with root word knowledge)

BioFacts 3.4 3/1 11. Diffusion = 12. Osmosis = 13. List the 3 solutions related to osmosis and describe each (make the cell shrink, swell, or stay the same.) 14. Passive transport (such as diffusion and osmosis) is the movement of molecules from: An area of high concentration to an area of low concentration An area of low concentration to an area of high concentration An area of equilibrium to an area of high concentration All of the above

BioFacts 3.5 3/2 15. Identify the solution as iso, hyper, or hypotonic and draw arrows labeling the direction of water movement. 16. A cell is placed into a salt solution. The salt concentration is greater in the solution than inside the cell. Which describes what will most likely happen? Water will flow into the cell by active transport Water will flow into the cell by passive transport Water will flow out of the cell by active transport Water will flow out of the cell by passive transport 30% solute 70% H2O 80% H2O 20 % solute 40% solute 85% H2O 15% solute 70% H2O

BioFacts 3.6 3/5 17. If energy is needed to move materials into or out of a cell, what is most likely occurring? Active transport Passive transport Osmosis Diffusion 18. During strenuous exercise, the body temperature increases. The body responds to the increase in temperature by sweating, which helps to reduce the body temperature. Which is demonstrated in this situation? Excretion Metabolism Homeostasis Synthesis