Volume 3, Issue 3, Pages 563-575 (May 2010) The Arabidopsis DSO/ABCG11 Transporter Affects Cutin Metabolism in Reproductive Organs and Suberin in Roots  Panikashvili David , Shi Jian Xin , Bocobza Samuel , Franke Rochus Benni , Schreiber Lukas , Aharoni Asaph   Molecular Plant  Volume 3, Issue 3, Pages 563-575 (May 2010) DOI: 10.1093/mp/ssp103 Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 1 DSO/ABCG11 Expression and Polar Protein Localization in Reproductive Organs. (A) Expression of pDSO/ABCG11:GFP in flower buds. (B) Expression of pDSO/ABCG11:GFP in open flower. Note the strong expression detected in the petal epidermis and the vasculature. (C) Expression of pDSO/ABCG11:GFP in developing seeds. (D) Expression of GFP:DSO/ABCG11 under the control of its native promoter in the walking-stick stage embryo (epidermal expression is marked by arrows). (E) Close-up view on GFP:DSO/ABCG11 expression under the control of its native promoter in the embryo epidermis. Note the distal mode of GFP expression (arrows). (F) Expression of GFP:DSO/ABCG11 under the control of the native promoter in the endosperm (en) tissue of the developing seed. In (D–F), the images were acquired through a GFP filter (I), chlorophyll filter (II), transmission (III) and (IV) was a merge between the GFP and chlorophyll filters. Molecular Plant 2010 3, 563-575DOI: (10.1093/mp/ssp103) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 2 Developmental Phenotypes of the dso-4 Mutant Lines. (A) Loss of apical dominance and stunted growth in flowering dso-4 as compared to a wild-type (WT) plant. (B) A defective flower of dso-4 compared to a WT flower. The hook-shaped silique in dso-4 is indicated by an arrow. (C) Semi-sterility in dso-4 as compared to a WT plant inflorescence. (D) Hook-shaped siliques are formed in dso-4 as compared to WT siliques. (E) SEM picture illustrating the surface of a hook-shaped silique in dso-4. The region of silique bending is indicated by arrows. (F) Fused seeds (indicated by an arrow) in the developing silique of dso-4. (G) Typical seeds formed in a developing silique of WT plant. Molecular Plant 2010 3, 563-575DOI: (10.1093/mp/ssp103) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 3 Transcriptome Changes in the Stems and Leaves of the dso-4 Plants. (A) A Venn diagram representing the number of transcripts that were down-regulated in either dso-4 plant leaves and stems or in both tissues. (B) A Venn diagram representing the number of transcripts that were up-regulated in either dso-4 plant leaves and stems or in both tissues. (C) Semi-quantitative RT–PCR expression analysis of selected transcripts in dso-4 and wild-type (WT) stems. (D) Semi-quantitative RT–PCR expressing analysis of selected transcripts in dso-4 and WT leaves. The UBIQ–UBIQUITINE gene served as a control for equal cDNA loading. Molecular Plant 2010 3, 563-575DOI: (10.1093/mp/ssp103) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 4 Altered Profile of Cutin Monomers in Flowers and Siliques of the dso-4 Lines. (A) The cutin polyester profile of dso-4 and wild-type (WT) flowers. (B) The cutin profile of dso-4 and WT siliques. Differential monomers are indicated by asterisks. The values indicate mean of three biological replicates ± stdev. * P < 0.05. Molecular Plant 2010 3, 563-575DOI: (10.1093/mp/ssp103) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 5 The Profile of Root Suberin Monomers in the dso-4 and Wild-Type Plants. Differential monomers are indicated by asterisks. The values indicate a mean of three biological replicates ± stdev. * P < 0.05. Molecular Plant 2010 3, 563-575DOI: (10.1093/mp/ssp103) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 6 Alteration to Cuticle and Suberin-Related Gene Expression in the Stem and Root Tissues of the dso-4 Lines. (A–E) Expression in stems. (F, G) Expression in roots. RQ, relative transcript level; the values indicate mean of three biological replicates ± SEM obtained by Quantitative RT–PCR analysis. * P < 0.05. See Table 1 for the annotation of encoded proteins. Molecular Plant 2010 3, 563-575DOI: (10.1093/mp/ssp103) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions