Trametinib and combination promote moDC maturation and decrease moDC viability. Trametinib and combination promote moDC maturation and decrease moDC viability.

Slides:

Advertisements

Similar presentations

Melanoma donor (MD) NK cells are functionally impaired/exhausted.

Advertisements

The CXC-chemokine platelet factor 4 promotes monocyte survival and induces monocyte differentiation into macrophages by Barbara Scheuerer, Martin Ernst,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation Regina K. Rowe, MD, PhD, David M. Pyle, MD,

Anti–4-1BB/PD-1 combination enriched CD8+ T cells in TILs

Uptake of T-MPs for DC maturation and antigen presentation.

CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell-stimulatory capability upon infection by.

Enterotoxin A presence in lung AMs and dendritic cells.

Experimental Hematology

Figure 3 Decreased AHI1 in human CD4+ T cells is associated with decreased proliferation and increased IFNγ production Decreased AHI1 in human CD4+ T cells.

Cetuximab treatment increases IFNγ receptor 1 expression.

Variable levels of cell surface and intracellular marker expression by HuT78 and HuT102 cells. Variable levels of cell surface and intracellular marker.

Suppressive effect of CD39+CD73+ melanoma cells on T-cell proliferation, and reversion of this effect via treatment with the CD39-blocking antibody, CD39.

Human Treg cells express PlexinB1.

Volume 140, Issue 1, Pages e3 (January 2011)

Increased chemokine content and leukocyte infiltrate in D6-negative tumors. Increased chemokine content and leukocyte infiltrate in D6-negative tumors.

The absence of ADCC by nivolumab in vitro.

Targeting allergen to FcγRI reveals a novel TH2 regulatory pathway linked to thymic stromal lymphopoietin receptor Kathryn E. Hulse, PhD, Amanda J. Reefer,

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

LV pseudotransduction delivers proteins and activates DCs.

T exosomes bind MAdCAM-1 via RA-increased integrin α4β7.

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

Human Beta-Defensin 3 Induces Maturation of Human Langerhans Cell–Like Dendritic Cells: An Antimicrobial Peptide that Functions as an Endogenous Adjuvant

Association of B cell TLR4 levels to disease activity.

CD8+ T cells were immunomodulated and required for the efficacy of anti–4-1BB/anti–PD-1 combination treatment. CD8+ T cells were immunomodulated and required.

ISS forming highly aggregated structures have decreased capability to induce maturation of human PDCs. Purified PDCs (105 cells) were stimulated with ISS.

The mucosal environment regulates CD160 expression on IELs

Effect of CD4+ Th1 cytokines and HER2-targeted antibodies on MHC class I restoration and HER2369–377-CD8+ T-cell targeting of HER2-expressing cancer cells.

Senescence-associated defective HLA-DR upregulation does not modulate immunosuppressive properties of MSCs. (A) Fit and senescent MSCs were subjected to.

MK-8628 suppresses T lymphocyte proliferation.

Toll-like receptor agonists differentially regulate cysteinyl-leukotriene receptor 1 expression and function in human dendritic cells Maryse Thivierge,

Exosome-mediated inhibition of T cells is reversible.

Dox titration and kinetic analyses of CD19CAR expression upon Dox administration and discontinuation. Dox titration and kinetic analyses of CD19CAR expression.

by Babs O. Fabriek, Robin van Bruggen, Dong Mei Deng, Antoon J. M

PD-L1 expression by DC subsets from lesional CTCL and healthy skin.

Dietary plant extracts inhibit NF-κB activation in vitro.

Anti-CD20 CAR mRNA enhances exPBNK in vitro cytolytic activity against CD20+ B-NHL cells and rituximab-resistant cells. exPBNK were electroporated in the.

Antigen-specific CD8+ T cells express higher levels of PD-1 in animals that received the optimized SSX2 vaccine. Antigen-specific CD8+ T cells express.

pDCs from the melanoma environment responded to TLR-L stimulation.

CLL-1 expression in patients with AML and normal donors.

CD8, galectin-3, galectin-9, and the M1/M2 ratio are associated with a longer survival. CD8, galectin-3, galectin-9, and the M1/M2 ratio are associated.

PD-1 inhibition stimulates the proliferation and cytokine secretion of exhausted/senescent CD8+ T cells in vitro. PD-1 inhibition stimulates the proliferation.

IL-13Rα2 promotes cell survival and proliferation.

Melanoma patient monocytes have altered expression of inflammatory and surface markers. Melanoma patient monocytes have altered expression of inflammatory.

by Fabian C. Verbij, Nicoletta Sorvillo, Paul H. P

Expression of XCR1 in normal ovarian surface epithelium and the IOSE and borderline EOC cell lines. Expression of XCR1 in normal ovarian surface epithelium.

MEDI4736 shows antitumor activity in xenograft mouse models of human cancer. MEDI4736 shows antitumor activity in xenograft mouse models of human cancer.

Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Uptake of TEX by DCs in vivo.

Canonical but not adaptive NK-cell function is suppressed by Tregs.

Effect of nivolumab or ipilimumab on immune responses to vaccination in cynomolgus monkeys. Effect of nivolumab or ipilimumab on immune responses to vaccination.

Expression of B7-H1, B7-DC, and PD-1 on B cells.

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

Monocytes from melanoma patients are unresponsive to TLR3 agonists.

Anti-CD40 activates TAMs and recruits inflammatory monocytes.

Human basophils are unresponsive to contact-dependent or contact-independent inhibition by Tregs. Human basophils are unresponsive to contact-dependent.

Combination of R848 and anti-CD200R affects activation of tumor-infiltrating myeloid cells. Combination of R848 and anti-CD200R affects activation of tumor-infiltrating.

Effect of bevacizumab on the proliferation of A2780 cells.

Typical pattern of the degranulation of NK cells from a healthy donor (upper row) and a patient with FRH in recurrence (middle row) and in remission (lower.

NE cleaves and activates GSDMD.

The effect of βAR signaling on the generation of a cytotoxic CD8+ T-cell response in vivo. The effect of βAR signaling on the generation of a cytotoxic.

Effect of HDC on the ROS production in mouse lungs after melanoma cell inoculation. Effect of HDC on the ROS production in mouse lungs after melanoma cell.

IL2Cx alone or in combination with anti–CTLA-4 increases the CD8/Treg ratio in the tumor. IL2Cx alone or in combination with anti–CTLA-4 increases the.

ARQ 531 is cytotoxic to CLL cells, decreases NF-κB function, and inhibits migration. ARQ 531 is cytotoxic to CLL cells, decreases NF-κB function, and inhibits.

MDSC population in patient peripheral blood.

EC-derived SP cells are targeted by CD30.CAR T cells.

RIL21 and checkpoint blockade restore IFNγ production in Tim-3+PD-1+ intratumoral NK cells from cancer patients. rIL21 and checkpoint blockade restore.

Surface expression of chemokine receptors on M

Expression of CAR, endogenous TCR, and target-cell receptors.

Trametinib and combination decrease T-lymphocyte proliferation.

Presentation transcript:

Trametinib and combination promote moDC maturation and decrease moDC viability. Trametinib and combination promote moDC maturation and decrease moDC viability. A, moDCs were incubated with the indicated concentrations of individual inhibitors or combinations, for 24 hours, in the presence of LPS. Cells were stained with fluorescently labeled live/dead stain, CD86, CD83, or anti–HLA-DR antibodies. MFI of bound antibody was determined by flow cytometry. Representative single-parameter histograms show the expression of CD80, CD83, and CD86 by MoDCs (gray) after the addition of LPS (dotted black line gray) or 30 nmol/L trametinib plus LPS (solid black line). B, moDCs were incubated with the indicated concentrations of individual inhibitors or combinations, for 24 or 48 hours. The toxicity exerted by the individual and combined inhibitors was assessed using a fluorescent live/dead dye. Mean values with SEM from 4 healthy donors are shown. One-way ANOVA with a Dunnett post test against the control was performed. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Dabrafenib, BRAFi; trametinib, MEKi. Laura J. Vella et al. Cancer Immunol Res 2014;2:351-360 ©2014 by American Association for Cancer Research