Lymphocyte proliferative responses of peripheral blood mononuclear cells to phytohemagglutinin (PHA) (A) as a function of plasma retinol concentration.

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Lymphocyte proliferative responses of peripheral blood mononuclear cells to phytohemagglutinin (PHA) (A) as a function of plasma retinol concentration (n = 7 VAD and n = 52 VAA children); (B) in boys with HbSS and VAD (n = 5) and boys with HbSS and VAA (n = 18); (C) in children with retinol binding protein (RBP) and plasma retinol below normal (n = 6) and children with normal plasma retinol but RBP below normal (n = 31); and (D) as a function of health scores (n = 29, n = 20, and n = 8 for health scores 0-2, 3-8, and >8, respectively). For 2A, 2B, and 2C, no significant differences in mean stimulation indexes (SI) were found between children with plasma retinol levels <20 μg/dL and those with levels ≥20 μg/dL. For 2D, for each PHA concentration (2.5, 5, 10, or 15 μg/mL), a dose-dependent decrease in the rate of DNA synthesis was seen with increased health scores (P < 0.05 to P = 0.1). Values are mean ± SEM. CPM, counts per minute; VAA, vitamin A–adequate, VAD, vitamin A–deficient. Lymphocyte proliferative responses of peripheral blood mononuclear cells to phytohemagglutinin (PHA) (A) as a function of plasma retinol concentration (n = 7 VAD and n = 52 VAA children); (B) in boys with HbSS and VAD (n = 5) and boys with HbSS and VAA (n = 18); (C) in children with retinol binding protein (RBP) and plasma retinol below normal (n = 6) and children with normal plasma retinol but RBP below normal (n = 31); and (D) as a function of health scores (n = 29, n = 20, and n = 8 for health scores 0-2, 3-8, and >8, respectively). For 2A, 2B, and 2C, no significant differences in mean stimulation indexes (SI) were found between children with plasma retinol levels <20 μg/dL and those with levels ≥20 μg/dL. For 2D, for each PHA concentration (2.5, 5, 10, or 15 μg/mL), a dose-dependent decrease in the rate of DNA synthesis was seen with increased health scores (P < 0.05 to P = 0.1). Values are mean ± SEM. CPM, counts per minute; VAA, vitamin A–adequate, VAD, vitamin A–deficient. Solo R. Kuvibidila et al. TOJ 2018;18:308-317 Copyright © 2018 by Ochsner Clinic Foundation