Fang Wang, Yunfeng Wang, Jie Ding, Jiyun Yang Kidney International

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Detection of mutations in the COL4A5 gene by analyzing cDNA of skin fibroblasts Fang Wang, Yunfeng Wang, Jie Ding, Jiyun Yang Kidney International Volume 67, Issue 4, Pages 1268-1274 (April 2005) DOI: 10.1111/j.1523-1755.2005.00204.x Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 1 Strategy for amplification of the COL4A5 cDNA by RT-PCR I: Schematic representation of the α5(IV) chain. NC1 represented noncollagenous carboxyl-terminal domain. II: Open boxes indicated the entire coding region of the α5(IV) chain mRNA, and the numbers in the boxes represented the exon numbers. The horizontal line indicated the 5′ and 3′ untranslated regions, respectively. The localizations of the five amplified fragments were shown with the primer site (arrow heads). Kidney International 2005 67, 1268-1274DOI: (10.1111/j.1523-1755.2005.00204.x) Copyright © 2005 International Society of Nephrology Terms and Conditions

Figure 2 RT-PCR amplification of the entire coding region of COL4A5 mRNA. M: DNA molecular mass marker; Lane A to E: Fragments A to E, respectively, PCR products obtained by the amplification of the COL4A5 cDNA. Kidney International 2005 67, 1268-1274DOI: (10.1111/j.1523-1755.2005.00204.x) Copyright © 2005 International Society of Nephrology Terms and Conditions