Tm-22 Confers Different Resistance Responses against Tobacco mosaic virus Dependent on Its Expression Level  Haili Zhang, Jinping Zhao, Shanshan Liu, Da-Peng Zhang, Yule Liu  Molecular Plant  Volume 6, Issue 3, Pages 971-974 (May 2013) DOI: 10.1093/mp/sss153 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 1 Tm-22 Expression Level Determines TMV Resistance Type in Transgenic Plants. AResponse of Tm-22-transformed N. benthamiana plants to TMV–GFP infection. Symptoms of local and systemic leaves in Tm-22 transgenic plants (TM#1, 34, and 74) and wild-type plants (WT) after TMV–GFP infection. Green fluorescence foci marked the TMV-expressed GFP. Left panels show the local leaves at 5 dpi. Right panels show the systemic leaves at 9 dpi. All leaves were photographed under white light and UV light. BRT–PCR was performed using TMV–GFP CP-specific primers to test the presence of TMV–GFP in local (5 dpi) and systemic leaves (9 dpi) of four plants (WT, TM#1, 34, and 74). CTm-22 mRNA levels in transgenic ER, HR, and SHR plants (TM#1, 5, 13, 20, 34, and 74) were analyzed by real-time RT–PCR using Tm-22-specific primer and normalized relative to the value of NbActin mRNA. ** P < 0.05; *** P < 0.01. Measurements were repeated three times respectively. The error bar indicates standard error. DResponse of Tm-22-silenced ER and HR plants to TMV–GFP infection. Systemic leaves of TM#1 (ER) and TM#34 (HR) Tm-22-silenced plants and respective TRV vector control plants were photographed at 20 dpi under white light and UV light. White arrows indicate SHR. ERT–PCR was performed using TMV–GFP CP-specific primers to confirm the presence of TMV–GFP in the systemic leaves of Tm-22-silenced ER and HR plants 20 dpi. TRV alone-infected control plants were used as negative control. FTm-22 mRNA levels in Tm-22-silenced ER and HR plants were analyzed by real-time RT–PCR using Tm-22-specific primers and normalized relative to the value of NbActin mRNA. Measurements were repeated three times respectively. The error bar indicates standard error. Three asterisks and two asterisks mark significant difference with P < 0.01. Molecular Plant 2013 6, 971-974DOI: (10.1093/mp/sss153) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions