Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and HAL-treated xenografts. Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and.

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Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and HAL-treated xenografts. Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and HAL-treated xenografts. Immunoblotting showed that hypoxia increased β-catenin protein levels in PLC/PRF/5 and HepG2 cells (A), and induced its intracellular translocation in Hep3B and MHCC97 cells (B). The induction of HIF-1α expression by hypoxia was detected in all cells with respect to their normoxic controls. Blots were normalized to β-actin protein in each lane. The ratios of each protein relative to matched normoxic cells are listed below each blot. C, immunoblotting of tumor tissues showed that HAL markedly increased HIF-1α expression in MHCC97-R and Hep3B-R xenografts compared with those in sham-operated groups but slightly upregulated β-catenin protein level. Ratios relative to corresponding sham-operated control were normalized to β-actin. D, immunostaining revealed that most β-catenin–positive cells in above tumor tissues showed a cytoplasmic/nuclear accumulation. Liang Liu et al. Clin Cancer Res 2010;16:2740-2750 ©2010 by American Association for Cancer Research