Metabolic profiling and characterization of reliance on de novo lipid synthesis of prostate cell lines. Metabolic profiling and characterization of reliance on de novo lipid synthesis of prostate cell lines. A, glucose uptake (left) and lactate (right) secretion in 3 prostate cancer cell lines (DU145, LNCaP, and PC3) and a nonmalignant prostate epithelial cell line (RWPE1). B, OCR of cells in the presence or absence of either 10 μM FCCP (gold bars) or 0.2 μg/mL oligomycin (purple bars). Total protein concentration was used for normalization. C,de novo pyruvate-dependent lipid synthesis rates of cells incubated in FM or LDM. D, glucose and glutamine dependency of cells incubated in FM or LDM. Induction of apoptosis was determined 72 hours after incubation in the indicated medium (CM, cell mass). E, cell mass assay after 72 hours of treatment with increasing concentrations of the fatty acid synthase inhibitor C75 (left) or the ATP-citrate lyase inhibitor SB204990 (right) in FM or LDM. The significance of the difference between FM and LDM was tested for the 2 greatest concentrations of each inhibitor. All graphs show mean and SD of 3 independent replicates (*P ≤ 0.05; **P ≤ 0.005, Student t test). Susana Ros et al. Cancer Discovery 2012;2:328-343 ©2012 by American Association for Cancer Research