Fig. 3 Electroporation-mediated RNAi.

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Fig. 3 Electroporation-mediated RNAi. Electroporation-mediated RNAi. (A) Measurement of knockdown effect using qRT-PCR. After injection of siRNA and electroporation, the epidermal samples were dissected at hour 12 of the fourth instar. Relative expressions of cll (n = 6), abd-A (n = 5), and Abd-B (n = 5) were measured. Student’s t test, *P < 0.05. (B) Injection of siRNA was performed at the end of the third instar, followed by observation during the fourth and fifth instars. Knockdown of cll was performed on the T3 segment (black dotted box). The blue dashed oval indicates the corresponding region of the eyespots in the fourth and fifth instars. The asterisk indicates that the complicated lines around it have changed after RNAi. The hollow arrowhead shows the si-cll side where the presumptive eyespot changed in size after RNAi. The filled arrowhead shows the control side where no phenotypic changes were detected. (C) Measurement of the red area in the E region after RNAi of cll. The cuticular samples were collected (n = 3), and the size for each region was measured using ImageJ. Student’s t test, *P < 0.05. Photo credit: H.J. H. Jin et al. Sci Adv 2019;5:eaav7569 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).