Sequence variation analysis of the prolactin receptor C-terminal region in women with premature ovarian failure Anne Bachelot, M.D., Ph.D., Justine Bouilly,

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Sequence variation analysis of the prolactin receptor C-terminal region in women with premature ovarian failure Anne Bachelot, M.D., Ph.D., Justine Bouilly, B.Sc., Yuchen Liu, B.Sc., Diane Rebourcet, Ph.D., Céline Leux, B.Sc., Frédérique Kuttenn, M.D., Philippe Touraine, M.D., Ph.D., Nadine Binart, Ph.D. Fertility and Sterility Volume 94, Issue 7, Pages 2772-2775 (December 2010) DOI: 10.1016/j.fertnstert.2010.06.040 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Activation of PI3 kinase pathways in ovaries of PRLR-/-RS mice. Ovaries from 8-week-old WT, PRLR-/-RS, and PRLR-/- mice were collected without and after 30 minutes of PRL stimulation and used for protein extraction. Western blot analysis was conducted for levels of activated mTOR, P-AKT, total AKT, activated S6K, total S6K, and tubulin as loading control in WT, PRLR-/-RS, and PRLR-/- ovaries. All experiments were repeated at least three times. Representative results are shown. Fertility and Sterility 2010 94, 2772-2775DOI: (10.1016/j.fertnstert.2010.06.040) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Expression of DPPA3 in WT, PRLR-/-RS, and PRLR-/- ovaries. Ovaries from of 9 PRLR-/-RS, 9 PRLR-/-, or 10 WT littermates (all 1 day old) were collected. Levels of dppa3 mRNA were measured by quantitative real-time polymerase chain reaction. The relative levels of dppa3 were normalized against levels of 18S. Relative expression within a given sample was calculated as the ratio (attomol of specific gene/fmol of 18S). The mRNA levels are shown as means ± SEM. Fertility and Sterility 2010 94, 2772-2775DOI: (10.1016/j.fertnstert.2010.06.040) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Exon 10 polymorphism in human PRLR gene. Schematic representation of the 11 exons of the human PRLR gene and corresponding protein domains; red arrows indicate alternative splicing sites. Exon 10 sense sequences obtained from one heterozygous patient harboring G-to-A mutated allele (variant) is shown as compared with the WT allele (control). Black arrows indicate the used primers for sequencing exon 10, representing the receptor long form (10F and 10R), and exon 11, representing the receptor short form (11F and 11R). TM = transmembrane domain. Fertility and Sterility 2010 94, 2772-2775DOI: (10.1016/j.fertnstert.2010.06.040) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions