Allergy multivaccines created by DNA shuffling of tree pollen allergens Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara.

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Presentation transcript:

Allergy multivaccines created by DNA shuffling of tree pollen allergens Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara Bohle, PhD, Lothar Vogel, PhD, Peter Briza, PhD, Heimo Breiteneder, PhD, Stefan Vieths, PhD, Arnulf Hartl, PhD, Adriano Mari, MD, Christof Ebner, MD, Peter Lackner, PhD, Peter Hammerl, PhD, Josef Thalhamer, PhD, Fatima Ferreira, PhD Journal of Allergy and Clinical Immunology Volume 120, Issue 2, Pages 374-380 (August 2007) DOI: 10.1016/j.jaci.2007.05.021 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Deduced amino acid sequence alignment of chimeric proteins TPC7 and TPC9 with 3 of 14 parental allergens used for DNA shuffling. Alignment was performed with ClustalW/EMBL-EBI. Dashes indicate identical amino acids. The immunodominant T-cell epitope of Bet v 1 is highlighted. Journal of Allergy and Clinical Immunology 2007 120, 374-380DOI: (10.1016/j.jaci.2007.05.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE binding of TPCs. A, ELISA with sera from patients with birch pollen allergy. Data are presented as mean OD405/492 units ± SD. P values were calculated by using paired-samples t tests (∗∗P < .01). B, Mediator release assay with RBL-2H3 cells. Protein concentrations (in nanograms per milliliter) to obtain 50% of maximum degranulation were determined. P values were calculated by using the Wilcoxon rank sum test (∗P < .05). Journal of Allergy and Clinical Immunology 2007 120, 374-380DOI: (10.1016/j.jaci.2007.05.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Immunogenicity of selected TPCs. A, Proliferative responses of PBMCs stimulated with parental allergens or chimeric proteins. Symbols represent individual patients, and bars represent medians. P values were calculated by using paired-samples t tests (∗P < .05). B, Proliferative responses of Bet v 1–specific TCLs A, B, and C to rBet v 1a, TPC7, or TPC9. Journal of Allergy and Clinical Immunology 2007 120, 374-380DOI: (10.1016/j.jaci.2007.05.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Genetic immunization. A, IgG responses after immunization with chimeric genes (up arrows) and sensitization with rBet v 1a (down arrows). B, Mediator release in RBL cells. P values were calculated with t tests (∗P < .05; ∗∗P < .01). C, ELISpot analysis. Data are expressed as mean cytokine-secreting cells per 106 spleen cells ± SEM. P values were calculated with t tests (∗P < .05). Journal of Allergy and Clinical Immunology 2007 120, 374-380DOI: (10.1016/j.jaci.2007.05.021) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions