Reading and interpretation of mCIM results.

Slides:

Advertisements

Similar presentations

Kirby-Bauer disk diffusion Testing skill based learning

Advertisements

Screening for new antibiotics

The Use of Cefoxitin for the Determination of Methicillin Resistance in Staphylococci John D. Perry Microbiology Department Freeman Hospital Newcastle.

Melioidosis: Current Diagnostic Approach and Role in Clinical practice Rob Baird Royal Darwin Hospital.

Nebraska Public Health Laboratory 2008 CLSI M100-S18 update Paul D. Fey, Ph.D. Associate Professor/Associate Director Josh Rowland, M.T. (ASCP) State Training.

Methods for detecting resistance Goal: To determine whether organism expresses resistances to agents potentially used for therapy Designed to determine.

The reading is 7.38 mm. The reading is 7.72 mm.

Antimicrobial Activity of Plant Extracts and the Resistance of Staphylococcus aureus and Escherichia coli to Tea Tree (Melaleuca alternifolia) Extract.

Chapter 42 Antimicrobial Sensitivity Testing

Can efflux confer high levels resistance to meropenem (MEM) in Pseudomonas aeruginosa (Pa) clinical isolates? H. Chalhoub1, H. Rodriguez-Villalobos2,

Antimicrobial Susceptibility Testing (AST)

Copyright © 2017 American Academy of Pediatrics.

Interpreting Exponential Functions

Antibiogram By:Dr. S. S. Khoramrooz In the name of God

نگهداری واستفاده از سویه های باکتریایی

Agar diffusion method Basmah almaarik

Survival of mice after infection with M

The evolution of antibiotic resistance associates with a mutation in an efflux pump. The evolution of antibiotic resistance associates with a mutation.

Scanning electron micrographs of a P

Itraconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

Antibiotic susceptibility testing

Lab# 8 Kirby-Bauer test BCH462 [practical].

MexT-associated downregulation of oprD expression.

Scanning electron micrographs of a P

Glucose concentration-dependent increase of melanin synthesis.

Zone of inhibition assay.

Relationships between H. pylori, inflammation, and acid secretion.

Positivity rates and concordance of multiplex PCR and blood culture (BC) results from 27 published studies. Positivity rates and concordance of multiplex.

Fluconazole tissue and fluid concentrations in humans as multiples of the maximal or simultaneously measured concentration in plasma (μg/ml) after systemic.

Number of outbreaks associated with drinking water by water system type and year (n = 780), 1971 to “Other” includes outbreaks associated with bottled.

Percentages of outbreak deficiencies (n = 671) in public water systems (n = 656) by time period, 1971 to 2006, excluding outbreaks associated with Legionella.

Susceptibility to hydrogen peroxide.

Recommended scheme for determination of heteroresistance and interpretation criteria. Recommended scheme for determination of heteroresistance and interpretation.

Number of outbreaks associated with drinking water by system type and month (n = 762), 1971 to 2006, excluding outbreaks associated with commercially bottled.

Growth of B. dermatitidis yeast is inhibited by deferoxamine only at concentrations of 0.5 mM or greater. Growth of B. dermatitidis yeast is inhibited.

Hector H. García et al. Clin. Microbiol. Rev. 2002; doi: /CMR

Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. Deletion of VdHapX renders Verticillium dahliae more susceptible to H2O2. (A)

Double-disk diffusion and Etest ESBL detection tests.

Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system. Amplification curve from the MeVA RT-qPCR on the Roche LightCycler 480 system.

API 20 E System & Antibiotic susceptibility test

Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500 platform. Specificity of the MeVA RT-qPCR assay, using an Applied Biosystems 7500.

The mexR and nalC mutations decrease C-30 inhibition of P

Multiplex PCR assay to detect common resistance determinants in B

Phylogenetic analyses showing the relationship between Ruminococcus, Blautia, and Clostridium on the basis of the sequences of the 16S region for each.

Recovery template. Recovery template. The recovery template (internal control) has the same sequence as the PCR product except the probe region has been.

Serial testing with IGRAs reveals underlying phenotypes.

Noncontrast computed tomography of patient C. B

Roberta B. Carey et al. Clin. Microbiol. Rev. 2018; doi: /CMR

Various possible courses of the immune response to severe sepsis and septic shock over 28 days. Various possible courses of the immune response to severe.

Western blot assay of an acute MIF-positive serum showing reactivity with the 135- and 115-kDa specific protein antigens of Rickettsia conorii and Rickettsia.

General schematic for the identification of bacteria and yeast by MALDI-TOF MS using the intact-cell method. General schematic for the identification of.

Schematic description of sites of action of different antifungal agents. Schematic description of sites of action of different antifungal agents. Candins.

Discriminatory patterns of B. cenocepacia (A) and B

Genotypic differentiation (G-based) for all pairs of populations.

(A) T. vaginalis parasite as seen in broth culture.

THE GROWTH ENVIRONMENT.

ABT-100 inhibits PI3K activity and p85 tyrosine phosphorylation.

NKT cell recognition of CD1d1 molecules is inhibited by treatment with ascites. NKT cell recognition of CD1d1 molecules is inhibited by treatment with.

Fluconazole zone diameter distributions for all Candida spp

TAE-684 effectively inhibits the growth of H3122 in vivo.

Outcomes of infections caused by carbapenemase-producing Klebsiella pneumoniae, according to treatment regimen. Outcomes of infections caused by carbapenemase-producing.

Phylogenetic tree of the complete genomes of 27 representative human (black) and camel (red) MERS-CoV strains rooted by NeoCoV (KC ). Phylogenetic.

Relationship of partial rpoB gene sequences inferred by the neighbor-joining method for Canadian study strains of C. pyruviciproducens. Relationship of.

Clinical presentation of Senecavirus A in sows.

The T3SS apparatus consists of rings that provide a continuous path across the inner (IM) and outer (OM) bacterial membranes, including the peptidoglycan.

Dendrogram relationships by 16S rRNA gene similarity of the A

Negative effect of the use of the SuperScript III Platinum One-Step quantitative RT-PCR kit on the specificity of the MeVA RT-qPCR for the vaccine genotype.

Dendrogram clustering the MALDI-TOF MSP obtained from at least 20 mass spectra of strains belonging to the C. haemulonii complex species and related species.

This figure shows the six phenotypes observed during CLI induction testing of S. aureus by disk diffusion. This figure shows the six phenotypes observed.

Example of population analysis profile curves for vancomycin-susceptible and heterogeneous vancomycin-intermediate S. aureus strains. Example of population.

Presentation transcript:

Reading and interpretation of mCIM results. Reading and interpretation of mCIM results. (A) A 22-mm zone of inhibition of growth of the carbapenem-susceptible indicator organism is present around the meropenem (MEM) disk that was incubated with the negative-control organism (N), while no zone of inhibition is present around the MEM disks that were incubated with the positive-control organism (P) or the test organism (T). (B) Closer examination of the zone around the MEM disk incubated with the negative-control organism reveals a narrow ring of growth abutting the disk, which represents carryover of the test organism from the tryptic soy broth; this growth is ignored when interpreting mCIM results. Virginia M. Pierce et al. J. Clin. Microbiol. 2017; doi:10.1128/JCM.00193-17