Tumor-specific panel of mAbs are reactive with human B7-H3.

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Tumor-specific panel of mAbs are reactive with human B7-H3. Tumor-specific panel of mAbs are reactive with human B7-H3. A, anti–B7-H3 mAb TES7 exhibits strong differential reactivity to multiple solid tumor tissues compared with normal tissues. No positive staining was noted in human normal pancreas, lung, liver, kidney, and heart. The very weak staining in the epithelium of the crypt of the colon was consistent with a nonspecific staining pattern. B, tandem mass spectrometry analysis of protein immunoprecipitated by GB8 mAb from A498 membrane lysate identifies B7-H3 as the potential antigen. TOF peptide matches are highlighted in bold. C, confirmation of reactivity of GB8 for human B7-H3 by ELISA using recombinant human B7-H3. D, anti–B7-H3 mAb BRCA84D exhibits strong differential reactivity to lung squamous carcinoma compared with normal adjacent lung tissue from the same patient specimen. Deryk Loo et al. Clin Cancer Res 2012;18:3834-3845 ©2012 by American Association for Cancer Research