The effects of AZD1775 and olaparib on DNA damage response signaling.

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Condition MiaPaCa-2 Control Gem (100nm) AZD1775 (200nM) Gem + AZD1775 Panc-1 Control Gem (50nm) AZD1775 (200nM) Gem + AZD1775 Capan-1 Control Gem (50nm)

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The effects of AZD1775 and olaparib on DNA damage response signaling. The effects of AZD1775 and olaparib on DNA damage response signaling. MiaPaCa-2 cells were treated with AZD1775 and olaparib for 1 hour pre- and 6 or 16 hours post-radiation (RT; 6 Gy). At the end of the drug treatment, cells were analyzed by immunoblotting for the indicated proteins. Data are from a single representative experiment (A) or are the mean fold-change in PAR or pCdk1 (Y15) levels relative to control ± SE at 16 hours post-radiation from 3 independent experiments (B and C). Statistical significance (P < 0.05) is indicated versus control* and radiation†. David Karnak et al. Clin Cancer Res 2014;20:5085-5096 ©2014 by American Association for Cancer Research