The heterotetrameric architecture of the epithelial sodium channel (ENaC) ENaC expressed at the cell surface is composed of a fixed number of α, β and.

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The heterotetrameric architecture of the epithelial sodium channel (ENaC) ENaC expressed at the cell surface is composed of a fixed number of α, β and γ subunits. (A and B) Xenopus oocytes were injected with 0.3 ng of cRNA of each FLAG‐tagged β and γ ENaC subunit (βF,γF) and different cRNA concentrations [0.3 (□), 1 (+), 3 (○), 10 (▵) and 30 (⋄) ng] of the non‐tagged α subunit. (A) A representative experiment showing the linear correlation between binding of [125I]anti‐FLAG antibodies to β and γ FLAG‐tagged subunits (fmol/oocyte) and the amiloride‐sensitive current INa measured in individual oocytes. (B) Ratios of specific antibody binding normalized for INa (fmol/μA) measured in individual oocytes over a wide range of injected α non‐tagged cRNA concentrations. Errors bars are means ± SE of three experiments performed with 12 oocytes per experimental condition. (C and D) Same experiments as in (A) and (B) but with oocytes injected with 0.3 ng of cRNA of FLAG‐tagged α and γ ENaC subunits (αF,γF) and increasing cRNA concentrations [0.3 (□), 1 (+), 3 (○), 10 (▵) and 30 (⋄) ng] of the non‐tagged β subunit. (E and F) Same experiments as in (A) and (B) but with oocytes injected with 0.3 ng of cRNA of FLAG‐tagged α and β ENaC subunits (αF,βF) and increasing cRNA concentrations [0.3 (□), 1 (+), 3 (○), 10 (▵) and 30 (⋄) ng] of the non‐tagged γ subunit. IF THIS IMAGE HAS BEEN PROVIDED BY OR IS OWNED BY A THIRD PARTY, AS INDICATED IN THE CAPTION LINE, THEN FURTHER PERMISSION MAY BE NEEDED BEFORE ANY FURTHER USE. PLEASE CONTACT WILEY'S PERMISSIONS DEPARTMENT ON PERMISSIONS@WILEY.COM OR USE THE RIGHTSLINK SERVICE BY CLICKING ON THE 'REQUEST PERMISSIONS' LINK ACCOMPANYING THIS ARTICLE. WILEY OR AUTHOR OWNED IMAGES MAY BE USED FOR NON-COMMERCIAL PURPOSES, SUBJECT TO PROPER CITATION OF THE ARTICLE, AUTHOR, AND PUBLISHER. EMBO J, Volume: 17, Issue: 2, Pages: 344-352, First published: 15 January 1998, DOI: (10.1093/emboj/17.2.344)