BCR/ABL expression, tyrosine phosphorylation, and signaling in dasatinib- and imatinib-resistant cell lines and the ubiquitin inhibitor lactacystin modifies.

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BCR/ABL expression, tyrosine phosphorylation, and signaling in dasatinib- and imatinib-resistant cell lines and the ubiquitin inhibitor lactacystin modifies the BCR/ABL protein. BCR/ABL expression, tyrosine phosphorylation, and signaling in dasatinib- and imatinib-resistant cell lines and the ubiquitin inhibitor lactacystin modifies the BCR/ABL protein. Protein tyrosine phosphorylation and abl (A), phosphorylation of Lyn, Lck, p21WAF, Akt, mitogen-activated protein kinase, actin (D) levels were analyzed by an immunoblotting protein (30 μg) from cell lysates. B, K562 BMS-R cells were treated with or without lactacystin for 24 h. Cell lysates were immunoprecipitated (IP) with anti-Abl Ab and immunoblotted (WB) with the ubiquitin or Abl Abs. C, K562 BMS-R cells were treated lactacystin for indicated hours. Cell viability was evaluated through the trypan blue exclusion and cell lysates were immunoblotted with Abl or actin Abs. Seiichi Okabe et al. Clin Cancer Res 2008;14:6181-6186 ©2008 by American Association for Cancer Research