GADD45 Regulates G2/M Arrest, DNA Repair, and Cell Death in Keratinocytes Following Ultraviolet Exposure Tomoko Maeda, Atef N. Hanna, Adrian B. Sim,

Slides:

Advertisements

Similar presentations

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation Stefan W. Stoll, Philip E. Stuart, Sylviane Lambert, Alberto.

Advertisements

Nucleotide Excision Repair Genes are Upregulated by Low-Dose Artificial Ultraviolet B: Evidence of a Photoprotective SOS Response? Tomoko Maeda, Prescillia.

Importance of Tissue Transglutaminase in Repair of Extracellular Matrices and Cell Death of Dermal Fibroblasts After Exposure to a Solarium Ultraviolet.

Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,

Sphingosylphosphorylcholine is a Potent Inducer of Intercellular Adhesion Molecule-1 Expression in Human Keratinocytes Genji Imokawa, Yutaka Takagi,

Volume 35, Issue 3, Pages (August 2009)

Requirement of heat shock protein 90 in mesangial cell mitogenesis

Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,

IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.

Phosphatidylinositol 3-Kinase/Akt-Dependent and -Independent Protection Against Apoptosis in Normal Human Melanocytes Masahiro Oka, Akiko Kageyama, Mizuho.

The DNA-Mismatch Repair Enzyme hMSH2 Modulates UV-B-Induced Cell Cycle Arrest and Apoptosis in Melanoma Cells Markus Seifert, Stefan J. Scherer, Wilfried.

Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34 Rebecca J. Morris, Carl D. Bortner, George.

Spleen Tyrosine Kinase Mediates EGFR Signaling to Regulate Keratinocyte Terminal Differentiation Nan-Lin Wu, Duen-Yi Huang, Li-Fang Wang, Reiji Kannagi,

Regulation of UVB-Induced IL-8 and MCP-1 Production in Skin Keratinocytes by Increasing Vitamin C Uptake via the Redistribution of SVCT-1 from the Cytosol.

Effects of XPD Mutations on Ultraviolet-Induced Apoptosis in Relation to Skin Cancer- Proneness in Repair-Deficient Syndromes Sophie Queille, Christiane.

Membrane Type 1 Matrix Metalloproteinase Regulates Cellular Invasiveness and Survival in Cutaneous Epidermal Cells Usha Nagavarapu, Kenneth Relloma,

Dermatopontin Regulates Fibrin Formation and Its Biological Activity

Decreased Growth Inhibitory Responses of Squamous Carcinoma Cells to Interferon-γ Involve Failure to Recruit cki Proteins into cdk2 Complexes Beth L.

Involvement of Fas (APO-1/CD-95) during Photodynamic-Therapy-Mediated Apoptosis in Human Epidermoid Carcinoma A431 Cells Nihal Ahmad, Sanjay Gupta, Denise.

Delphinidin, an Anthocyanidin in Pigmented Fruits and Vegetables, Protects Human HaCaT Keratinocytes and Mouse Skin Against UVB-Mediated Oxidative Stress.

Green Tea Polyphenols Prevent Ultraviolet Light-Induced Oxidative Damage and Matrix Metalloproteinases Expression in Mouse Skin Praveen K. Vayalil, Anshu.

Enhanced Death Ligand-Induced Apoptosis in Cutaneous SCC Cells by Treatment with Diclofenac/Hyaluronic Acid Correlates with Downregulation of c-FLIP

Cell-Density-Dependent Regulation of Expression and Glycosylation of Dopachrome Tautomerase/Tyrosinase-Related Protein-2 Thomas J. Hornyak, Daniel J.

Shalini S. Tibudan, Yihua Wang, Mitchell F. Denning

Protein Phosphatase Type 1-Dependent Dephosphorylation of the Retinoblastoma Tumor Suppressor Protein in Ultraviolet-Irradiated Human Skin and Keratinocytes

P38 Mitogen-Activated Protein Kinase Inhibitor Protects the Epidermis Against the Acute Damaging Effects of Ultraviolet Irradiation by Blocking Apoptosis.

Davina A. Lewis, Simone F. Hengeltraub, Feng C. Gao, Megan A

Trichothiodystrophy Fibroblasts Are Deficient in the Repair of Ultraviolet-Induced Cyclobutane Pyrimidine Dimers and (6–4)Photoproducts Yoko Nishiwaki,

Ultraviolet B Irradiation Induces Expansion of Intraepithelial Tumor Cells in a Tissue Model of Early Cancer Progression Norbert E. Fusenig Journal.

Role of p38 MAPK in UVB-Induced Inflammatory Responses in the Skin of SKH-1 Hairless Mice Arianna L. Kim, Jeffrey M. Labasi, Yucui Zhu, Xiuwei Tang,

Ginsenoside F1 Protects Human HaCaT Keratinocytes from Ultraviolet-B-Induced Apoptosis by Maintaining Constant Levels of Bcl-2 Enn Hee Lee, Si Young.

COP1 Contributes to UVB-Induced Signaling in Human Keratinocytes

Olga M. Mazina, Marjorie A. Phillips, Trevor Williams, Carol A

Volume 35, Issue 3, Pages (August 2009)

Shiou-Hwa Jee Journal of Investigative Dermatology

The p53-Stabilizing Compound CP Enhances Ultraviolet-B-Induced Apoptosis in a Human Melanoma Cell Line MMRU Yvonne Luu, Gang Li, Dr Journal of.

Supranuclear Melanin Caps Reduce Ultraviolet Induced DNA Photoproducts in Human Epidermis Nobuhiko Kobayashi, Akemi Nakagawa, Tsutomu Muramatsu, Yukio.

Staurosporine-Induced Cleavage of α-Smooth Muscle Actin During Myofibroblast Apoptosis Ayako Nakazono-Kusaba, Fumi Takahashi-Yanaga, Sachio Morimoto,

P38 Mitogen-Activated Protein Kinase Mediates Dual Role of Ultraviolet B Radiation in Induction of Maturation and Apoptosis of Monocyte-Derived Dendritic.

FRAP DNA-Dependent Protein Kinase Mediates a Late Signal Transduced from Ultraviolet-Induced DNA Damage Daniel B. Yarosh, Nicholas Bizios, Jeannie Kibitel,

Raymond L. Warters, Patrick J. Adamson, Christopher D. Pond, Sancy A

Paul J. Hensbergen, Astrid E. Alewijnse, Johanna Kempenaar, Rose C

Role for Protein Kinase C-α in Keratinocyte Growth Arrest

Characterization of Keratinocyte Differentiation Induced by Ascorbic Acid: Protein Kinase C Involvement and Vitamin C Homeostasis1 Isabella Savini, Antonello.

Resistance of Human Melanoma Cells Against the Death Ligand TRAIL Is Reversed by Ultraviolet-B Radiation via Downregulation of FLIP Elke Zeise, Michael.

Yu-Ying He, Jian-Li Huang, Michelle L. Block, Jau-Shyong Hong, Colin F

Adenosine Receptors as Mediators of Both Cell Proliferation and Cell Death of Cultured Human Melanoma Cells Stefania Merighi, Prisco Mirandola, Daniela.

Gang Li, Jason A. Bush, Vincent C. Ho

Differential Roles of Insulin Receptor and Insulin-Like Growth Factor-1 Receptor in Differentiation of Murine Skin Keratinocytes Efrat Wertheimer, Meirav.

Targeted Depletion of Polo-Like Kinase (Plk) 1 Through Lentiviral shRNA or a Small- Molecule Inhibitor Causes Mitotic Catastrophe and Induction of Apoptosis.

Interferon-γ-Mediated Growth Regulation of Melanoma Cells: Involvement of STAT1- Dependent and STAT1-Independent Signals Anja Bosserhoff Journal of Investigative.

Tumor necrosis factor-α and lipopolysaccharide induce apoptotic cell death in bovine glomerular endothelial cells Udo K. Meßmer, Verena A. Briner, Josef.

James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber

Differential Responses of S100A2 to Oxidative Stress and Increased Intracellular Calcium in Normal, Immortalized, and Malignant Human Keratinocytes Tong.

Infrared Radiation Confers Resistance to UV-Induced Apoptosis Via Reduction of DNA Damage and Upregulation of Antiapoptotic Proteins Christian Jantschitsch,

IL-23 Antagonizes UVR-Induced Immunosuppression through Two Mechanisms: Reduction of UVR-Induced DNA Damage and Inhibition of UVR-Induced Regulatory T.

A p38MAPK/HIF-1 Pathway Initiated by UVB Irradiation Is Required to Induce Noxa and Apoptosis of Human Keratinocytes Kris Nys, An Van Laethem, Carine.

ΔNp63α Promotes Apoptosis of Human Epidermal Keratinocytes

1α,25-Dihydroxyvitamin D3 Stimulates Activator Protein 1 DNA-Binding Activity by a Phosphatidylinositol 3-Kinase/Ras/MEK/Extracellular Signal Regulated.

Hidetoshi Takahashi, Akemi Ishida-Yamamoto, Hajime Iizuka

ATR–Chk1 Pathway Inhibition Promotes Apoptosis after UV Treatment in Primary Human Keratinocytes: Potential Basis for the UV Protective Effects of Caffeine

Keratinocyte Apoptosis Induced by Ultraviolet B Radiation and CD95 Ligation – Differential Protection through Epidermal Growth Factor Receptor Activation.

Galectin-3 Protects Keratinocytes from UVB-Induced Apoptosis by Enhancing AKT Activation and Suppressing ERK Activation Jun Saegusa, Daniel K. Hsu, Wei.

Journal of Investigative Dermatology

CD40 Ligation Alters the Cell Cycle of Differentiating Keratinocytes

Thiol Antioxidants Block the Activation of Antigen-Presenting Cells by Contact Sensitizers Stefanie Bruchhausen, Sabine Zahn, Elke Valk, Jürgen Knop,

Deon G. Uffort, Elizabeth A. Grimm, Julie A. Ellerhorst

Nerve Growth Factor Protects Human Keratinocytes from Ultraviolet-B-Induced Apoptosis Alessandra Marconi, Cristina Vaschieri, Silvia Zanoli, Alberto.

Ultraviolet-B-Induced G1 Arrest is Mediated by Downregulation of Cyclin-Dependent Kinase 4 in Transformed Keratinocytes Lacking Functional p53 Arianna.

Volume 22, Issue 3, Pages (May 2006)

Presentation transcript:

GADD45 Regulates G2/M Arrest, DNA Repair, and Cell Death in Keratinocytes Following Ultraviolet Exposure Tomoko Maeda, Atef N. Hanna, Adrian B. Sim, Prescillia P. Chua, Michelle T. Chong, Victor A. Tron, Dr Journal of Investigative Dermatology Volume 119, Issue 1, Pages 22-26 (July 2002) DOI: 10.1046/j.1523-1747.2002.01781.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Gadd45a–/– cells demonstrate massive cell death and G2/M arrest cannot be sustained. Gadd45a+/+ or Gadd45a–/– cells were irradiated with 50 J per m2 UVB and harvested; floating cells were included in the analysis. Cells were fixed, stained with propidium iodide, and analyzed by flow cytometry. The sub-G0/G1 population was calculated using CELLQuest software as the percent apoptotic cells was determined. G0/G1, G2/M population was assessed with ModifitLT program. The numbers in each panel reflect sub-G0/G1, G0/G1, and G2/M populations (left to right), and are representative of one of our independent experiments. Experiments were repeated at least four times with similar results. Journal of Investigative Dermatology 2002 119, 22-26DOI: (10.1046/j.1523-1747.2002.01781.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 GADD45 regulates Cdc2 kinase activity. Cdc2 kinase assay was performed with cell lysates from Gadd45a+/+ or –/– keratinocytes. Cells were irradiated at 50 J per m2 or unirradiated, and harvested at different time points. Cell lysate (400 µg) was incubated with Cdc2 antibody for 5 h at 4°C. After the recovery of the immunocomplex, the kinase reaction was initiated with histone H1 by adding 10 µCi of [32P]ATP at 30°C. Gels were visualized by autography, and the extent of phosphorylation of histone 1 was measured by liquid scintillation counting of the histone-H1-containing gel slice. Cdc2 kinase activity in UV-irradiated Gadd45a+/+ cells (black bars). Cdc2 kinase activity in UV-irradiated Gadd45a–/– cells (white bars). The experiments were repeated three times and the result represents the mean ± SD. Lower panel is shown as a representative result of the autoradiography. Journal of Investigative Dermatology 2002 119, 22-26DOI: (10.1046/j.1523-1747.2002.01781.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 GADD45 regulates Cdc2 nuclear transport after UV. Cdc2 localization after UV exposure was examined with indirect immunofluorescence. Growing Gadd45a+/+ or –/– cells on a coverslip were irradiated at 50 J per m2 UVB or unirradiated, and fixed 6 h after irradiation. Cdc2 was detected with the antibody and the binding was visualized by fluorescein-isothiocyanate-conjugated secondary antibody. (a) Cdc2 localization of unirradiated Gadd45a–/– cells. (b) Cdc2 localization of UV-irradiated Gadd45a–/– cells. (c) Cdc2 localization of unirradiated Gadd45a+/+ cells. (d) Cdc2 localization of UV-irradiated Gadd45a+/+ cells. Journal of Investigative Dermatology 2002 119, 22-26DOI: (10.1046/j.1523-1747.2002.01781.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Thymine dimer repair is deficient in Gadd45a–/– keratinocytes. DNA repair activity of Gadd45a+/+ or –/– cells was assessed by slot-Western analysis. The cells were irradiated at 25, 50, or 100 J per m2 UVB, and the DNA was harvested 0, 8, 24, or 48 h after irradiation. Thymine dimers were quantified in DNA samples from irradiated cells using a dimer-specific monoclonal antibody, and the repair rate was calculated. Values were normalized for difference in DNA sample loading. DNA repair activity of Gadd45a+/+ cells (solid circle). DNA repair activity of Gadd45a–/– cells (open circle). Journal of Investigative Dermatology 2002 119, 22-26DOI: (10.1046/j.1523-1747.2002.01781.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Gadd45a-deficient cells are more sensitive to UV than GADD45a-proficient cells. UV sensitivity of Gadd45a+/+ cells or Gadd45a–/– cells was assessed using crystal violet assay. Cells were irradiated with UVB when they reached 80% confluency and were stained with crystal violet 48 h after irradiation. Absorbance at 570 nm was measured with a spectrophotometer and the relative density was calculated, estimating that in the absence of UV irradiation 100% relative density is expected. Gadd45a+/+ cells (solid circle), GADD45a–/– cells (open circle). The experiment was repeated three times with similar results. The D37 for Gadd45a+/+ and Gadd45a–/– cells was 100 and 58 J per m2, respectively. Journal of Investigative Dermatology 2002 119, 22-26DOI: (10.1046/j.1523-1747.2002.01781.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions