scrambled si-CLOCK scrambled si-CLOCK scrambled si-CLOCK scrambled si-CLOCK Supplemental Figure 2. The mRNA expression of putative CLOCK-regulated genes before or after CLOCK knockdown. The cells were treated with scrambled control or CLOCK siRNAs and harvested every 4 h. The mRNA expression of each gene was analyzed by qRT-PCR. The data are presented as the mean ± SD of three independent experiments. Scrambled, scrambled siRNA; si-CLOCK, CLOCK siRNA; THBS1, thrombospondin1; LAMTOR1, late endosomal/lysosomal adaptor, MAPK and MTOR activator 1; SIK1, salt-inducible kinase 1; and RIOK3, RIO kinase 3.