Snail-induced multinucleation follows midbody persistence. Snail-induced multinucleation follows midbody persistence. A, Persistent midbodies (arrowheads) are visible between sister SCp2 mouse mammary epithelial cells that later merge to form a multinucleated cell. Images extracted from timelapse microscopy. Scale bars, 10 μm. See also Supplementary Movie S1 and S2. B, MBDs in cells ectopically expressing Snail are quantified by MKLP1-positive puncta. Red, MKLP1; green, Snail-GFP; blue, DNA. Top row, scale bars, 50 μm. Bottom row, scale bars, 10 μm. Arrows in bottom row, MBDs. C, A higher fraction of multinucleated cells ectopically expressing Snail have MBDs than mononucleated cells expressing Snail. D, Multinucleated cells accumulate more MBDs than do mononucleated cells. This difference is exacerbated in cells expressing Snail as compared with those expressing GFP alone. Cells expressed GFP or Snail for 48 hours. Shown are mean ± SEM for three experiments (n > 500 cells for C and D). *, P < 0.05; ***, P < 0.001 (C, paired t test; D, two-way ANOVA). Allison K. Simi et al. Cancer Res 2018;78:2277-2289 ©2018 by American Association for Cancer Research