Fig. 6 Pig 5 tissue development.

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Fig. 6 Pig 5 tissue development. Pig 5 tissue development. Pig 5 developed a partial airway occlusion after transplantation. Bronchoscopy images of (A) carina, (B) bronchial occlusion (arrow) and open airway, and (C) image of bronchial occlusion alone (black arrow) in BEL. (D) Anastomosis site of BEL in recipient’s trachea. (E) Chest x-ray of the nonaerated BEL, seen as the dense homogeneous opacity, projecting over the mediastinum. Extensive compensatory hyperinflation of the native lung occurred. (F) CT image of chest in venous phase through the left hemithorax showing collapsed BEL containing multiple small intercostal vessels (arrows). (G, I, and L) DAPI-stained controls and BEL in (G and H) aerated and (J to N) nonaerated regions containing P-SPC+ (red) and TUNEL+ (green) AEC II. (H) P-SPC+ (red) cells with inset of enlarged image. (J and K) BEL in nonaerated region cells stained for P-SPC+ (red) and TUNEL+ (green), a marker indicative of cells undergoing apoptosis. (L) DAPI-stained control and (M and N) FSP-1+ (red) fibroblasts in nonaerated regions. Five randomly selected areas from 10 different sections of tissue immunostained were examined for TUNEL+ or FSP-1+ cells in native lung and in aerated or nonaerated sections of BEL. (O) Averaged number of cells, number of P-SPC+ AEC II, TUNEL+ AEC II, and FSP-1+ cells ± SD are shown for native lung and aerated and nonaerated BEL. Data were analyzed using ANOVA. *P < 0.05, **P < 0.005, ***P < 0.0005. NS, not significant. Joan E. Nichols et al., Sci Transl Med 2018;10:eaao3926 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works