Single nucleotide polymorphism array analysis can distinguish different genetic mechanisms that lead to loss of heterozygosity (LOH). Single nucleotide.

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Presentation transcript:

Single nucleotide polymorphism array analysis can distinguish different genetic mechanisms that lead to loss of heterozygosity (LOH). Single nucleotide polymorphism array analysis can distinguish different genetic mechanisms that lead to loss of heterozygosity (LOH). A, different chromosomal mechanisms can cause LOH. In this cartoon, a mutated tumor suppressor gene indicated by a red star become homozygous because the remaining wild-type allele is lost by a copy-neutral event such as recombination or gene conversion (top right) or by hemizygous deletion (bottom right). B and C, LOH analysis (top left), copy number analysis (bottom left), and copy number quantitation of chromosome 13 in cell lines HCC1599 and HCC2218. In the LOH panel, yellow denotes heterozygosity (AB), whereas red (AA) and blue (BB) denote homozygosity. Note that LOH regions show heterozygous SNP markers in the normal that are reduced to homozygosity in the cell line. D and E, LOH within chromosome 9 associated with copy number loss and an interstitial homozygous deletion in NCI-H1648, contrasted with LOH and copy number maintenance in HCC1187. Xiaojun Zhao et al. Cancer Res 2004;64:3060-3071 ©2004 by American Association for Cancer Research