AKT activation in HCC2429 is SRC- but not Notch-dependent.

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AKT activation in HCC2429 is SRC- but not Notch-dependent. AKT activation in HCC2429 is SRC- but not Notch-dependent. A, HCC2429 cells were treated with rapamycin (20 nmol/L) alone or together with Notch inhibitor DAPT (15 μmol/L) for 24 hours in the presence or absence of serum, and cell lysates were analyzed by immunoblotting. Note that HES1 is not seen in cells treated with DAPT. pAKT(S473) shows no major change in response to DAPT. B, HCC2429 cells were treated with Notch inhibitor compound E (comp E) at the indicated doses for 24 hours in the presence or absence of serum. Note that HES1 levels are absent in cells treated with compound E but there is no effect on pAKT(S473) levels. C, cells were treated with Notch inhibitor DAPT or compound E for 48 hours at the indicated doses. Cell numbers were determined by the MTT assay and normalized to untreated cells. D and E, HCC2429 cells were treated with SRC inhibitor PP2 or dasatinib at the indicated doses for 24 hours in the absence (D) or presence (E) of serum. F, cells were treated with SRC inhibitor PP2 or dasatinib for 48 hours at the indicated doses. Cell numbers were determined by the MTT assay and normalized to untreated cells. Yanan Guo et al. Mol Cancer Res 2013;11:282-293 ©2013 by American Association for Cancer Research