Microbiology Chapter 3, Quiz #1 Review

Format 40 Formal Points 1 ordering question (5 points) 15 Multiple Choice Questions (1 point each) Multi part open ended question where you will go through the process of creating and analyzing a bacterial growth curve like on the graphing activity packet (20 points)

Vocabulary to know… Five I’s – Know in Order!!! Inoculation Incubation Isolation Inspection Identification *Also be able to describe each step. Agar Media Specific Media Non-Specific Media Sterile Technique Colony Pure Culture Mixed Culture Contaminated Culture

Test Tube Growth Clear Turbid Flocculent Pellicle Sediment

Sterile Technique What are some of the issues that may result when sterile technique is not followed carefully?

What is the easiest way to tell if bacteria grown is harmful or not?

Inspection vs. Identification What are the main differences between inspection and identification?

Articles What is Growing on an 8 year old’s hands? What makes good bacteria go bad? *You will be able to use your yellow sheet of notes to help you on these questions, so we will NOT recap this. The article links are posted on my schoolwires so you may go back and read / review these.

Bacterial Growth Plates We did our fingerprints. Even though nobody wants to have a “dirty” finger, why would not producing any growth for an unwashed finger actually be a bad thing? How does color, shape and texture indicate what type of microorganism may be growing?

Bacterial Growth Curves Phases Lag Exponential Stationary Death For each phase know: How contagious How symptomatic If antibiotics should be prescribed Be able to: Highlight a key, graph and table Determine rate of growth or death Determine number of days spent in each phase Analyze data

Any Questions????