Volume 9, Issue 1, Pages 182-186 (January 2016) The Sensor Histidine Kinases AHK2 and AHK3 Proceed into Multiple Serine/Threonine/Tyrosine Phosphorylation Pathways in Arabidopsis thaliana  Rebecca Dautel, Xu Na Wu, Michael Heunemann, Waltraud X. Schulze, Klaus Harter  Molecular Plant  Volume 9, Issue 1, Pages 182-186 (January 2016) DOI: 10.1016/j.molp.2015.10.002 Copyright © 2016 The Author Terms and Conditions

Figure 1 The AHK2/AHK3-Dependent Two-Component Signaling System Causes Ser/Thr/Tyr Phosphorylation and Dephosphorylation in Arabidopsis thaliana. (A) Functional categorization of all identified phosphoproteins in the dataset (n =836), the phosphoproteins differentially modified between the ahk2ahk3 mutant and wild type (WT) after 10 min of kinetin (100 ng/ml) treatment (n =150; kinetin dataset), with separation of higher (n = 78) and lower (n = 72) phosphorylation in the mutant, as well as the phosphoproteins differentially modified between the ahk2ahk3 mutant and wt in the absence of kinetin (n = 34). Diameters of the pie chart also represent the numbers of peptides included. The category “other” is composed of proteins with unknown function and proteins of the non-specified categories. Exploded pie slices marked with asterisks represent significantly over-represented functional categories (p < 0.01, Fisher exact test). (B–E) Significant (p < 0.05, t-test) log2-fold phosphorylation changes of ahk2ahk3 mutant versus WT within the kinetin dataset in proteins with function in cytokinin metabolism and signaling (B), calcium signaling (C), as protein kinases (D), or in cell organization (E). Blue bars indicate higher phosphorylation in the ahk2ahk3 mutant; red bars indicate higher phosphorylation in WT. Error bars represent SD of two replica experiments, and multiple testing correction was applied. (F) Schematic drawings of the domain structure of AHK2 and ARR7 according to the SMART database. Phosphorylation sites identified in this study are indicated by red letters P; pink letter P marks phosphorylation sites identified in other studies. Green bar indicates coiled-coil region and magenta bar indicates region of low complexity. Molecular Plant 2016 9, 182-186DOI: (10.1016/j.molp.2015.10.002) Copyright © 2016 The Author Terms and Conditions