Phosphate Binding in the Active Site of Alkaline Phosphatase and the Interactions of 2- Nitrosoacetophenone with Alkaline Phosphatase-Induced Small Structural.

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Presentation transcript:

Phosphate Binding in the Active Site of Alkaline Phosphatase and the Interactions of 2- Nitrosoacetophenone with Alkaline Phosphatase-Induced Small Structural Changes Le Zhang, René Buchet, Gérard Azzar Biophysical Journal Volume 86, Issue 6, Pages 3873-3881 (June 2004) DOI: 10.1529/biophysj.103.034116 Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 1 Reaction scheme for the photolysis of caged ATP and the hydrolysis of ATP by intestinal alkaline phosphatase. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 2 Reaction-induced infrared difference spectra of intestinal alkaline phosphatase induced by the photorelease of ATP in 2H2O. Reaction-induced infrared difference spectra in the 1800–1300cm−1 region. (Trace i) RIDS of caged ATP without AP. (Trace ii) RIDS of caged ATP with IAP. (Trace iii) Corrected RIDS of caged ATP with IAP corresponding to the difference: spectrum ii minus spectrum i. The corrected RIDS reflected structural changes caused by binding to IAP after photorelease of ATP from its cage and sequential ATP hydrolysis by IAP. Buffer composition was 100mM Tris-HCl, p2H=7.0, 10mM MgCl2, and 5mM caged ATP with or without 10mg/ml (0.09mM) IAP. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 3 Reaction-induced infrared difference spectra of intestinal alkaline phosphatase produced by the photorelease of ATP in 2H2O. Reaction infrared difference spectra in the phosphate region. (Trace i) RIDS of caged ATP without IAP. (Trace ii) RIDS of caged ATP with IAP. Buffer composition was 100mM Tris-HCl, p2H=7.0, 10mM MgCl2 and 5mM caged ATP with or without 10mg/ml (0.09mM) IAP. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 4 Determination of magnitude of structural changes of intestinal alkaline phosphatase caused by the photorelease of ATP in 2H2O and its hydrolysis. Trace i is the infrared spectrum of caged ATP with IAP before photolysis. Trace ii is the corrected RIDS of caged ATP with IAP. Its absorbance scale was expanded 20 times comparatively to the scale for the upper spectrum. For both traces, the buffer composition was 100mM Tris-HCl, p2H=7.0, 10mM MgCl2, and 5mM NPE-caged ATP with 10mg/ml (0.09mM) IAP. The calculated intensity of IR spectrum (trace i), corresponding to A, and the calculated intensity of RIDS (trace ii) corresponding to ΔA, are indicated by shaded surface. The ΔA/2A ratio, in the 1760–1600cm−1 region, permitted the determination of the magnitude of peptide backbone structural change of IAP induced by the photorelease of ATP and its hydrolysis. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 5 Reaction-induced infrared difference spectra of intestinal alkaline phosphatase induced by the photorelease of ATP in H2O. Reaction-induced infrared difference spectra in the 1800–900cm−1 region. Trace i is the RIDS of NPE-caged ATP without IAP; trace ii is the RIDS of NPE-caged ATP with IAP; trace iii is the corrected RIDS of NPE-caged ATP with IAP corresponding to the difference: spectrum ii minus spectrum i. Buffer composition was 200mM Tris-HCl, pH=7.4, 10mM MgCl2, and 20mM NPE-caged ATP with or without 50mg/ml (0.45mM) IAP. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 6 Comparison of reaction-induced infrared difference spectra of intestinal alkaline phosphatase produced by the photorelease of ATP from NPE-caged ATP in H2O and in 2H2O buffers. (Trace i) Corrected RIDS of NPE-caged ATP with IAP in H2O buffer. Buffer composition was 200mM Tris-HCl, pH=7.4, 10mM MgCl2, and 20mM NPE-caged ATP with 50mg/ml (0.45mM) IAP. The residual 1687cm−1 peak (indicated by a star) corresponds probably to the carbonyl group of the photoproduct, 2-nitrosoacetophenone. (Trace ii) Corrected RIDS of NPE-caged ATP with IAP in 2H2O buffer. Buffer composition was 100mM Tris-HCl, p2H=7.0, 10mM MgCl2, and 5mM NPE-caged ATP with 10mg/ml (0.09mM) IAP. The absorbance scales of the spectrum i has been expanded two times comparatively to the scale for the spectrum ii. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 7 Reaction-induced infrared difference spectra of intestinal alkaline phosphatase produced by the photorelease of ATP. Effects of the increase of the NPE-caged ATP concentration on the structure of IAP. Trace A is the RIDS of 10mM caged ATP with IAP. Trace B is the RIDS of 7.5mM caged ATP with IAP. Trace C is the RIDS of 5mM caged ATP with IAP. Trace D is the RIDS of 2.5mM caged ATP with IAP. All the RIDS were corrected for the contribution of photolysis of NPE-caged ATP in the infrared spectra (see Fig. 2). Buffer composition was 100mM Tris-HCl, p2H=7.0, 10mM MgCl2, and 10mg/ml (0.09mM) IAP with the indicated concentrations of NPE-caged ATP. Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Figure 8 Reaction-induced infrared difference spectra of intestinal alkaline phosphatase produced by the photorelease of ATP. Effects of Pi and adenosine on the structure of IAP. Trace A is the RIDS of IAP without adenosine in Tris-HCl buffer (5mM caged ATP with 10mg/ml (0.09mM) IAP in buffer containing 100mM Tris-HCl, p2H=7.0, and 10mM MgCl2). Trace B is the RIDS of IAP in phosphate buffer (5mM caged ATP with 10mg/ml (0.09mM) IAP in buffer containing 100mM phosphate, p2H=7.0, 10mM MgCl2). Trace C is the RIDS of IAP in the presence of adenosine (5mM caged ATP with 10mg/ml (0.09mM) IAP and 5mM adenosine in buffer containing 100mM Tris-HCl, p2H=7.0, 10mM MgCl2). Trace D is the RIDS of IAP in the presence of adenosine and phosphate (5mM caged ATP with 10mg/ml IAP and 5mM adenosine in buffer containing 100mM phosphate, p2H=7.0, 10mM MgCl2). Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions

Scheme 1 Biophysical Journal 2004 86, 3873-3881DOI: (10.1529/biophysj.103.034116) Copyright © 2004 The Biophysical Society Terms and Conditions