Decreased dopamine uptake following downregulation of SLC6A3.

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Decreased dopamine uptake following downregulation of SLC6A3. Decreased dopamine uptake following downregulation of SLC6A3. A, Relative expression of SLC6A3 mRNA after downregulation of SLC6A3 in SNU-349 using two different siRNAs. Expression was normalized to siNeg in each experiment, and statistical significance was calculated compared with siNeg ctrl. B, [3H]-dopamine uptake of cells transfected with siNeg ctrl or two different siSLC6A3. Uptake was performed as described in Fig. 4A, 48 hours after transfection. Data; mean ± SEM. mRNA levels were related to housekeeping genes SDHA, UBC, and YWHAZ. Statistical significance was calculated using two-tailed Student t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Jennifer Hansson et al. Clin Cancer Res 2017;23:2105-2115 ©2017 by American Association for Cancer Research