HOXA9 and STAT5 co-occupy similar genomic regions and increase JAK/STAT signaling. HOXA9 and STAT5 co-occupy similar genomic regions and increase JAK/STAT.

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HOXA9 and STAT5 co-occupy similar genomic regions and increase JAK/STAT signaling. HOXA9 and STAT5 co-occupy similar genomic regions and increase JAK/STAT signaling. A, Volcano plot of differential gene expression analysis of age-matched CD8+ T-ALL JAK3M511I/HOXA9 vs. JAK3M511I-only leukemia cells. B, JAK3M511I/HOXA9 leukemia ChIP-seq density heat maps centered on STAT5 signal alongside HOXA9 and associated H3K27Ac, H3K27me3, H3K4me3, and H3K4me1 histone marks. C, Frequency of STAT5/HOXA9 co-occupied DNA regions with promoters (H3K4me3-positive) and enhancers (H3K27Ac-positive/H3K4me3-negative). D, Individual ChIP-seq tracks for canonical STAT5 target genes Pim1 and Bcl2. E, GSEA analysis of RNA-seq differentially expressed genes for JAK3M511I/HOXA9 vs. JAK3M511I and loss of the repressive H3K27me3 chromatin marks or gain in H3K27ac activation mark. F, Transcription factor loading of STAT5 and HOXA9 in upregulated genes, downregulated genes, or genes that do not change. G, Heat map and GSEA pathway of JAK/STAT signaling pathway genes in CD8+ control T-cell vs. JAK3M511I vs. JAK3M511I/HOXA9 leukemia. Charles E. de Bock et al. Cancer Discov 2018;8:616-631 ©2018 by American Association for Cancer Research