Impaired efferocytosis in human chronic granulomatous disease is reversed by pioglitazone treatment Ruby F. Fernandez-Boyanapalli, PhD, Emilia Liana.

Slides:

Advertisements

Similar presentations

Joint modeling of parentally reported and physician-confirmed wheeze identifies children with persistent troublesome wheezing Danielle C.M. Belgrave, MSc,

Advertisements

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

D-dimer: A biomarker for antihistamine-resistant chronic urticaria

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Pharmacologic rationale for treating allergic and nonallergic rhinitis

Alexandra Kazaks, MA, RD, Janet Y. Uriu-Adams, PhD, Judith S

Increased phenotypic differentiation and reduced corticosteroid sensitivity of fibrocytes in severe asthma Chun-Yu Lo, MD, Charalambos Michaeloudes,

Pioglitazone restores phagocyte mitochondrial oxidants and bactericidal capacity in chronic granulomatous disease Ruby F. Fernandez-Boyanapalli, PhD,

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Defective nuclear entry of hydrolases prevents neutrophil extracellular trap formation in patients with chronic granulomatous disease Susana Romao, PhD,

Obesity impairs apoptotic cell clearance in asthma

Long-term immune reconstitution after matched unrelated hematopoietic stem cell transplantation for immunodeficiency Vy Hong-Diep Kim, MD, Brenda Reid,

Is 9 more than 2 also in allergic airway inflammation?

Jonathan A. Bernstein, MD, David M. Lang, MD, David A. Khan, MD

Functional FCGR2B gene variants influence intravenous immunoglobulin response in patients with Kawasaki disease Sadeep Shrestha, PhD, Howard W. Wiener,

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Clinical studies of the DP1 antagonist laropiprant in asthma and allergic rhinitis George Philip, MD, Janet van Adelsberg, MD, Thomas Loeys, PhD, Nancy.

The National Biome Initiative: An allergy perspective

Physician needs in health informatics: Just ask the docs

Gut microbiome variations during hematopoietic stem cell transplant in severe combined immunodeficiency Jonathan P. Lane, MBBS, Christopher J. Stewart,

Treatment of chronic autoimmune urticaria with omalizumab

Nonadherence to Asthma Treatment: Getting Unstuck

Peter M. Wolfgram, MD, David B. Allen, MD

William J. Calhoun, MD, Tmirah Haselkorn, PhD, Dave P

β-Agonist enhances type 2 T-cell survival and accumulation

Roel P. Gazendam, MD, Annemarie van de Geer, MD, John L

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

News & Notes Journal of Allergy and Clinical Immunology

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

Daphne Koinis-Mitchell, PhD, Timothy Craig, DO, Cynthia A

A cluster-randomized trial shows telephone peer coaching for parents reduces children's asthma morbidity Jane M. Garbutt, MB, ChB, Yan Yan, MD, PhD,

Invasive fungal disease in autosomal-dominant hyper-IgE syndrome

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS

Sharon Seth, MD, David A. Khan, MD

What is an “eosinophilic phenotype” of asthma?

Onset of action of pimecrolimus cream 1% in the treatment of atopic eczema in infants Roland Kaufmann, MD, Regina Fölster-Holst, MD, Peter Höger, MD,

Cephalosporin Side Chain Cross-reactivity

Medication use in children with asthma in Finland from 1995 to 2006

Direct ex vivo analysis of allergen-specific CD4+ T cells

Granulomatous-lymphocytic Interstitial Lung Disease in a Patient with Common Variable Immunodeficiency Phil Lieberman, MD, John Routes, MD The Journal.

H. William Kelly, PharmD Journal of Allergy and Clinical Immunology

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Advances in the approach to the patient with food allergy

Geographic variability in childhood asthma prevalence in Chicago

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

Alexandra Kazaks, MA, RD, Janet Y. Uriu-Adams, PhD, Judith S

Biology of nasal polyposis

Efficacy of nasal mometasone for the treatment of chronic sinonasal disease in patients with inadequately controlled asthma Anne E. Dixon, MA, BM BCh,

Pınar Uysal, MD, Esben Eller, MSc, PhD, Charlotte G

Guus A. Westerhof, MD, Elise M. Vollema, MD, Els J

Neil E. Alexis, PhD, John C. Lay, PhD, Martha Almond, RRT, David B

Reply Journal of Allergy and Clinical Immunology

Macrolide antibiotics and asthma treatment

Asthma: The past, future, environment, and costs

Cigarette smoke exposure is associated with vitamin D3 deficiencies in patients with chronic rhinosinusitis Jennifer K. Mulligan, PhD, Whitney Nagel,

Janet Chou, MD, Maxim Lutskiy, MD, PhD, Erdyni Tsitsikov, PhD, Luigi D

Functional assessment of pathogenic IgG subclasses in chronic autoimmune urticaria Sridharan Soundararajan, PhD, Yoko Kikuchi, DVM, Kusumam Joseph, PhD,

Environmental factors and eosinophilic esophagitis

News Beyond Our Pages Journal of Allergy and Clinical Immunology

Effect of codeine on objective measurement of cough in chronic obstructive pulmonary disease Jaclyn Smith, MD, PhD, Emily Owen, MPhil, John Earis, MD,

Natural history of cow’s milk allergy

Primary prevention of asthma and allergy

Itching as a systemic disease

Chitinases and chitinase-like proteins in TH2 inflammation and asthma

Presentation transcript:

Impaired efferocytosis in human chronic granulomatous disease is reversed by pioglitazone treatment Ruby F. Fernandez-Boyanapalli, PhD, Emilia Liana Falcone, MD, Christa S. Zerbe, MD, Beatriz E. Marciano, MD, S. Courtney Frasch, PhD, Peter M. Henson, DVD, PhD, Steven M. Holland, MD, Donna L. Bratton, MD Journal of Allergy and Clinical Immunology Volume 136, Issue 5, Pages 1399-1401.e3 (November 2015) DOI: 10.1016/j.jaci.2015.07.034 Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Impaired efferocytosis by CGD monocytes is restored by pioglitazone treatment ex vivo. A, Freshly isolated monocytes from patients with CGD and normal (NL) subjects were “fed” fluorescently labeled apoptotic Jurkat cells (left) or carboxylated beads (right) (N = 9 subjects/group). B, Overnight-shipped CGD and normal monocytes, plated and treated for 24 hours with pioglitazone or vehicle, were then “fed” carboxylated beads as in Fig 1, A (N = 5 subjects/group). pio, Pioglitazone. Journal of Allergy and Clinical Immunology 2015 136, 1399-1401.e3DOI: (10.1016/j.jaci.2015.07.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Efferocytosis is restored and mitochondrial oxidant production enhanced in monocytes isolated from patients with CGD during treatment with pioglitazone. A, Efferocytosis by CGD monocytes appears to be restored (compared with normal monocytes) after treatment with pioglitazone for at least 30 days in 2 subjects with CGD. Patient data are shown as mean ± range for duplicate wells at each time point. B, Mitochondrial oxidant production by stimulated CGD monocytes was enhanced during treatment with pioglitazone. ctr, Control; MFI, mean fluorescence index; NL, normal; pio, pioglitazone; pre, before; Pt, patient. Journal of Allergy and Clinical Immunology 2015 136, 1399-1401.e3DOI: (10.1016/j.jaci.2015.07.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 A, Carboxylated beads taken up by monocytes in efferocytosis assays (magnification 40×). B, Phagocytosis of heat-killed Candida by normal (NL) and CGD monocytes is not different. Journal of Allergy and Clinical Immunology 2015 136, 1399-1401.e3DOI: (10.1016/j.jaci.2015.07.034) Copyright © 2015 American Academy of Allergy, Asthma & Immunology Terms and Conditions