In silico taxon coverage.

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In silico taxon coverage. In silico taxon coverage. The average in silico taxonomic coverage estimates obtained with PrimerProspector for the selected forward primers from the literature [designated “(L)”] and newly designed forward primers [designated “(N)”]. The published ITS1 (L) primer was recommended by UNITE and is therefore shown in this figure despite the low coverage performance. Coverage estimates are based on 5,789 simulated species in the SIS database. Coverage estimates are based on the default alignment criteria of PrimerProspector. Mykhaylo Usyk et al. mSphere 2017; doi:10.1128/mSphere.00488-17