The primary axis scores of each sample from a constrained ordination of principal coordinates (CAP) for the temperature profile of each city are indicated via color coding on a U.S. map. The primary axis scores of each sample from a constrained ordination of principal coordinates (CAP) for the temperature profile of each city are indicated via color coding on a U.S. map. Scores to the left on the ordination are plotted in shades of blue, and scores to the right on the ordination are plotted in shades of red. Samples that are ≥2 standard deviations from the primary axis origin are colored the maximum blue and red colors. Both the human fecal oligotype (bottom) and non-human-fecal oligotype (top) data sets were included in the CAP, and therefore, colors represent equivalent community variation related to the temperature profile of the cities for each data set. For comparison, sample periods are depicted in separate maps. Ryan J. Newton et al. mBio 2015; doi:10.1128/mBio.02574-14