Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock communities. Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock.

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Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock communities. Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock communities. Relative abundances of expected taxa are labeled with the corresponding taxonomic level from sequencing results. “Amplicon” represents the 16S rRNA PCR amplicon mock community, and “gDNA” represents the gDNA mock community. The results shown were analyzed following the DADA2 pipeline with the Greengenes database. Each bar represents the mean ± SD from three replicates. Proportions for each community were compared to expected proportions using ANOVA with Bonferroni’s multiple-comparison test. P values of <0.05 were considered to be significantly different from the expected values and are indicated by an asterisk above each bar plot. Zhengyao Xue et al. mSphere 2018; doi:10.1128/mSphere.00410-18